| Label | Region | Length (bp) | Pi | Alignment reliability | MarkerSeek score | Primer available | Action |
|---|---|---|---|---|---|---|---|
| rps16-trnQ-UUG | LSC | 1607 | 0.0134 | 0.99 | 67.3 | yes | View details |
| psbK-psbI | LSC | 406 | 0.0217 | 0.99 | 70.1 | yes | View details |
| trnS-GCU-trnG-UCC | LSC | 731 | 0.0127 | 0.97 | 66.7 | yes | View details |
| trnS-UGA-psbZ | LSC | 342 | 0.0376 | 1.00 | 74.9 | yes | View details |
| trnT-UGU-trnL-UAA | LSC | 976 | 0.0134 | 1.00 | 74.3 | yes | View details |
| ndhC-trnV-UAC | LSC | 1162 | 0.0134 | 1.00 | 62.0 | yes | View details |
| accD-psaI | LSC | 834 | 0.0185 | 0.97 | 64.0 | yes | View details |
| petA-psbJ | LSC | 1047 | 0.0149 | 0.97 | 75.6 | yes | View details |
| ycf1 | IRb | 1092 | 0.0023 | 1.00 | 49.5 | no | View details |
| rpl32-trnL-UAG | SSC | 886 | 0.0136 | 0.99 | 66.9 | yes | View details |
| ndhA | SSC | 2229 | 0.0065 | 0.99 | 56.5 | yes | View details |
| ycf1 | SSC | 5661 | 0.0087 | 1.00 | 48.0 | yes | View details |
| atpF-atpH | LSC | 396 | 0.0161 | 0.99 | 77.2 | yes | View details |
| trnK-UUU-rps16 | LSC | 605 | 0.0126 | 0.99 | 72.5 | yes | View details |
| trnT-GGU-psbD | LSC | 1482 | 0.0073 | 1.00 | 72.5 | yes | View details |
| trnG-UCC-trnR-UCU | LSC | 269 | 0.0143 | 1.00 | 68.5 | yes | View details |
| rpoB-trnC-GCA | LSC | 1221 | 0.0062 | 1.00 | 66.8 | yes | View details |
| ndhF-rpl32 | SSC | 822 | 0.0092 | 1.00 | 64.1 | yes | View details |
| psbE-petL | LSC | 1334 | 0.0086 | 1.00 | 63.2 | yes | View details |
| petN-psbM | LSC | 1169 | 0.0085 | 1.00 | 62.7 | yes | View details |
| trnC-GCA-petN | LSC | 748 | 0.0096 | 1.00 | 60.7 | yes | View details |
| Candidate markers include all hotspot labels marked in the diversity plot, listed in genomic order, plus the top 10 remaining regions by MarkerSeek score. Primer design is attempted for every listed region. Primer available: yes means at least one valid primer pair was found; no means no valid pair passed screening; NA means primer design was skipped. | |||||||
Species
27
Genome length
158–159 kb
Candidate markers
266
Primer pairs
100
Genome-wide nucleotide diversity
Candidate markers
12 hotspot labels from the diversity plot in genomic order, plus the top 9 remaining regions by MarkerSeek score (out of 266 candidates).
Primer pairs
Showing the top 30 of 100 primer pairs (ranked by primer score).
| Primer ID | Label | Forward | Reverse | Amplicon (bp) | Cross-species rate | Score |
|---|---|---|---|---|---|---|
| trnK-UUU-rps16_p1 | trnK-UUU-rps16 | GGACGAATCCCTTGCTTCAT |
GGTGCTCAACCTACAGAAACT |
765–800 | 1.000 | 78.2 |
| trnK-UUU-rps16_p2 | trnK-UUU-rps16 | ATGGACGAATCCCTTGCTTC |
GGTGCTCAACCTACAGAAACT |
767–802 | 1.000 | 78.1 |
| trnK-UUU-rps16_p3 | trnK-UUU-rps16 | CGATGGTATGGACGAATCCC |
GGTGCTCAACCTACAGAAACT |
774–809 | 1.000 | 77.8 |
| trnK-UUU-rps16_p4 | trnK-UUU-rps16 | CGAATCCCTTGCTTCATCCA |
GGTGCTCAACCTACAGAAACT |
762–797 | 0.926 | 74.8 |
| trnK-UUU-rps16_p5 | trnK-UUU-rps16 | ACGAATCCCTTGCTTCATCC |
GGTGCTCAACCTACAGAAACT |
763–798 | 0.926 | 74.8 |
| rps16-trnQ-UUG_p1 | rps16-trnQ-UUG | GTCTTTCAAGTCGCACGTTG |
GAGGTTCGAATCCTTCCGTC |
1567–1794 | 1.000 | 89.0 |
| rps16-trnQ-UUG_p2 | rps16-trnQ-UUG | ATGTCTTTCAAGTCGCACGT |
GAGGTTCGAATCCTTCCGTC |
1569–1796 | 1.000 | 88.0 |
| rps16-trnQ-UUG_p3 | rps16-trnQ-UUG | AAGTCGCACGTTGCTTTCTA |
GAGGTTCGAATCCTTCCGTC |
1560–1787 | 1.000 | 88.0 |
| rps16-trnQ-UUG_p4 | rps16-trnQ-UUG | CATGTCTTTCAAGTCGCACG |
GAGGTTCGAATCCTTCCGTC |
1570–1797 | 1.000 | 87.3 |
| rps16-trnQ-UUG_p5 | rps16-trnQ-UUG | TGTCTTTCAAGTCGCACGTT |
GAGGTTCGAATCCTTCCGTC |
1568–1795 | 1.000 | 86.3 |
| psbK-psbI_p1 | psbK-psbI | CAAGCTGCCGTAAGTTTTCG |
TCTTCACGTCCAGGATTACG |
522–542 | 1.000 | 81.0 |
| psbK-psbI_p2 | psbK-psbI | AGCTGCCGTAAGTTTTCGAT |
TCTTCACGTCCAGGATTACG |
520–540 | 1.000 | 80.7 |
| psbK-psbI_p3 | psbK-psbI | AAGCTGCCGTAAGTTTTCGA |
TCTTCACGTCCAGGATTACG |
521–541 | 1.000 | 80.4 |
| psbK-psbI_p4 | psbK-psbI | GCTGCCGTAAGTTTTCGATG |
TCTTCACGTCCAGGATTACG |
519–539 | 1.000 | 80.2 |
| psbK-psbI_p5 | psbK-psbI | GCAAGCTGCCGTAAGTTTTC |
TCTTCACGTCCAGGATTACG |
523–543 | 1.000 | 78.9 |
| trnS-GCU-trnG-UCC_p1 | trnS-GCU-trnG-UCC | ATTAGCAATCCGCCGCTTTA |
AACCGAAAGACCCCTTAACT |
801–876 | 0.963 | 73.4 |
| trnS-GCU-trnG-UCC_p2 | trnS-GCU-trnG-UCC | ACGGAAAGAGAGGGATTCGA |
AACCGAAAGACCCCTTAACT |
850–925 | 0.963 | 72.2 |
| trnS-GCU-trnG-UCC_p3 | trnS-GCU-trnG-UCC | ATTAGCAATCCGCCGCTTTA |
AAACCGAAAGACCCCTTAACT |
802–877 | 0.963 | 71.5 |
| trnS-GCU-trnG-UCC_p4 | trnS-GCU-trnG-UCC | GCTTTAGTCCACTCAGCCAT |
AACCGAAAGACCCCTTAACT |
787–862 | 0.963 | 70.5 |
| trnS-GCU-trnG-UCC_p5 | trnS-GCU-trnG-UCC | ACGGAAAGAGAGGGATTCGA |
AAACCGAAAGACCCCTTAACT |
851–926 | 0.963 | 70.3 |
| trnG-UCC-trnR-UCU_p1 | trnG-UCC-trnR-UCU | AGCCTTCCAAGCTAACGATG |
AGAAGACCTCTGTCCTATCCA |
341–384 | 1.000 | 71.1 |
| trnG-UCC-trnR-UCU_p2 | trnG-UCC-trnR-UCU | CCTAGCCTTCCAAGCTAACG |
AGAAGACCTCTGTCCTATCCA |
344–387 | 1.000 | 70.9 |
| trnG-UCC-trnR-UCU_p3 | trnG-UCC-trnR-UCU | AGCCTTCCAAGCTAACGATG |
AGGTTTAGAAGACCTCTGTCCT |
347–390 | 1.000 | 70.4 |
| trnG-UCC-trnR-UCU_p4 | trnG-UCC-trnR-UCU | CCTAGCCTTCCAAGCTAACG |
AGGTTTAGAAGACCTCTGTCCT |
350–393 | 1.000 | 70.2 |
| trnG-UCC-trnR-UCU_p5 | trnG-UCC-trnR-UCU | CCCTAGCCTTCCAAGCTAAC |
AGAAGACCTCTGTCCTATCCA |
345–388 | 1.000 | 68.7 |
| atpF-atpH_p1 | atpF-atpH | ATTAAACCCGAAACTCCCGG |
CGGAGGGAAAAATACGAGGT |
569–619 | 1.000 | 83.6 |
| atpF-atpH_p2 | atpF-atpH | ATTAAACCCGAAACTCCCGG |
CGAGGCGGAGGGAAAAATAC |
574–624 | 1.000 | 83.4 |
| atpF-atpH_p3 | atpF-atpH | ATTAAACCCGAAACTCCCGG |
GAGGCGGAGGGAAAAATACG |
573–623 | 1.000 | 83.4 |
| atpF-atpH_p4 | atpF-atpH | ATTAAACCCGAAACTCCCGG |
GCGGAGGGAAAAATACGAGG |
570–620 | 1.000 | 83.4 |
| atpF-atpH_p5 | atpF-atpH | ATTAAACCCGAAACTCCCGG |
GGCGGAGGGAAAAATACGAG |
571–621 | 1.000 | 83.4 |
Result downloads
Reference species (27)
One GenBank record per species. Use the NCBI button to open the Nucleotide entry for the listed accession.
| Species | Accession | Length (bp) | Link |
|---|---|---|---|
| Styrax acuminatus | PV083600.1 | 158100 | View on NCBI ↗ |
| Styrax aureus | PV083601.1 | 158441 | View on NCBI ↗ |
| Styrax chinensis | NC_057967.1 | 158502 | View on NCBI ↗ |
| Styrax duclouxii | NC_059790.1 | 157913 | View on NCBI ↗ |
| Styrax gentryi | PV083602.1 | 158022 | View on NCBI ↗ |
| Styrax glabrescens | PV083603.1 | 157923 | View on NCBI ↗ |
| Styrax hainanensis | PV083604.1 | 157950 | View on NCBI ↗ |
| Styrax jaliscanus | PV083605.1 | 158030 | View on NCBI ↗ |
| Styrax latifolius | PV083606.1 | 158176 | View on NCBI ↗ |
| Styrax leprosus | PV083607.1 | 158227 | View on NCBI ↗ |
| Styrax maninul | PV083608.1 | 158452 | View on NCBI ↗ |
| Styrax martii | PV083609.1 | 158120 | View on NCBI ↗ |
| Styrax nunezii | PV083610.1 | 158408 | View on NCBI ↗ |
| Styrax obtusifolius | PV083611.1 | 158641 | View on NCBI ↗ |
| Styrax officinalis | PV083612.1 | 158458 | View on NCBI ↗ |
| Styrax pedicellatus | PV083613.1 | 158424 | View on NCBI ↗ |
| Styrax pentlandianus | PV083614.1 | 158332 | View on NCBI ↗ |
| Styrax peruvianus | PV083615.1 | 158361 | View on NCBI ↗ |
| Styrax platanifolius | PV083616.1 | 158530 | View on NCBI ↗ |
| Styrax pohlii | PV083617.1 | 158304 | View on NCBI ↗ |
| Styrax portoricensis | PV083618.1 | 158426 | View on NCBI ↗ |
| Styrax ramirezii | NC_041138.1 | 158315 | View on NCBI ↗ |
| Styrax shiraianus | PV083619.1 | 157793 | View on NCBI ↗ |
| Styrax subargenteus | PV083620.1 | 158396 | View on NCBI ↗ |
| Styrax suberifolius | NC_041125.1 | 158480 | View on NCBI ↗ |
| Styrax tomentosus | PV083621.1 | 158589 | View on NCBI ↗ |
| Styrax warscewiczii | PV083622.1 | 158416 | View on NCBI ↗ |