Hotspot

ndhC-trnV-UAC

LSC · 53184-54345 · 1162 bp

Back to Styrax

Pi 0.0134
MarkerSeek score 62.00
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1162
variable_sites
81
pi
0.0134
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGGGCTAAAACTCCGGAAAT AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 1038-1333 bp 1.0 86.3
ndhC-trnV-UAC_p2 CTGGTCCTTTGCTAATCGGG AGAAGGTCTACGGTTCGAGT 55.0 50.0 58.3 57.8 1055-1350 bp 1.0 84.7
ndhC-trnV-UAC_p3 CCTTTGCTAATCGGGGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.7 57.8 1050-1345 bp 1.0 84.6
ndhC-trnV-UAC_p4 GGGGCTAAAACTCCGGAAAT GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.9 57.5 1037-1332 bp 1.0 83.6
ndhC-trnV-UAC_p5 TCCTTTGCTAATCGGGGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.5 57.8 1051-1346 bp 1.0 83.4