Hotspot

rpoB-trnC-GCA

LSC · 27652-28872 · 1221 bp

Back to Styrax

Pi 0.00624
MarkerSeek score 66.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
1221
variable_sites
54
pi
0.00624
unique_haplotype_count
23
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 CGTCAAGCCCTGATCAATGA AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.0 58.4 1194-1374 bp 1.0 84.3
rpoB-trnC-GCA_p2 CGTCAAGCCCTGATCAATGA GCGACACCCAGATTTGAACT 50.0 50.0 58.0 58.5 1219-1399 bp 1.0 83.2
rpoB-trnC-GCA_p3 AGAAAGCTCTTCCGTCAAGC AAAAGGATTTGCAGTCCCCC 50.0 50.0 58.2 58.4 1206-1386 bp 0.962963 81.3
rpoB-trnC-GCA_p4 CGTCAAGCCCTGATCAATGA TTTGTTGATCAGGCGACACC 50.0 50.0 58.0 58.8 1231-1411 bp 1.0 80.9
rpoB-trnC-GCA_p5 AGAAAGCTCTTCCGTCAAGC GCGACACCCAGATTTGAACT 50.0 50.0 58.2 58.5 1231-1411 bp 0.962963 80.3