Hotspot

atpF-atpH

LSC · 13831-14226 · 396 bp

Back to Styrax

Pi 0.0161
MarkerSeek score 77.20
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
396
variable_sites
42
pi
0.0161
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG CGGAGGGAAAAATACGAGGT 50.0 50.0 57.9 57.3 569-619 bp 1.0 83.6
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG CGAGGCGGAGGGAAAAATAC 50.0 55.0 57.9 58.7 574-624 bp 1.0 83.4
atpF-atpH_p3 ATTAAACCCGAAACTCCCGG GAGGCGGAGGGAAAAATACG 50.0 55.0 57.9 58.7 573-623 bp 1.0 83.4
atpF-atpH_p4 ATTAAACCCGAAACTCCCGG GCGGAGGGAAAAATACGAGG 50.0 55.0 57.9 58.7 570-620 bp 1.0 83.4
atpF-atpH_p5 ATTAAACCCGAAACTCCCGG GGCGGAGGGAAAAATACGAG 50.0 55.0 57.9 58.7 571-621 bp 1.0 83.4