Hotspot

trnS-GCU-trnG-UCC

LSC · 9013-9743 · 731 bp

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Pi 0.0127
MarkerSeek score 66.70
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
731
variable_sites
43
pi
0.0127
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.97
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 ATTAGCAATCCGCCGCTTTA AACCGAAAGACCCCTTAACT 45.0 45.0 58.0 56.4 801-876 bp 0.962963 73.4
trnS-GCU-trnG-UCC_p2 ACGGAAAGAGAGGGATTCGA AACCGAAAGACCCCTTAACT 50.0 45.0 58.2 56.4 850-925 bp 0.962963 72.2
trnS-GCU-trnG-UCC_p3 ATTAGCAATCCGCCGCTTTA AAACCGAAAGACCCCTTAACT 45.0 42.9 58.0 57.1 802-877 bp 0.962963 71.5
trnS-GCU-trnG-UCC_p4 GCTTTAGTCCACTCAGCCAT AACCGAAAGACCCCTTAACT 50.0 45.0 57.6 56.4 787-862 bp 0.962963 70.5
trnS-GCU-trnG-UCC_p5 ACGGAAAGAGAGGGATTCGA AAACCGAAAGACCCCTTAACT 50.0 42.9 58.2 57.1 851-926 bp 0.962963 70.3