Hotspot

accD-psaI

LSC · 61774-62607 · 834 bp

Pi 0.0185

MarkerSeek score 64.00

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 71
pi: 0.0185
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTGACAGCCAGTTTTGGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1090-1210 bp	1.0	89.2
accD-psaI_p2	CTACTACTGGTGGGGTGACA	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1103-1223 bp	1.0	88.8
accD-psaI_p3	TACTACTGGTGGGGTGACAG	CCATTGCAATTGCCGGAAAT	55.0	45.0	58.1	58.0	1102-1222 bp	1.0	88.8
accD-psaI_p4	TGATCGATTCAGGCACTTGG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1520-1640 bp	1.0	88.1
accD-psaI_p5	TTCAGGCACTTGGGATCCTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	1513-1633 bp	1.0	88.1