Hotspot

rpl32-trnL-UAG

SSC · 116952-117837 · 886 bp

Back to Styrax

Pi 0.0136
MarkerSeek score 66.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
886
variable_sites
59
pi
0.0136
unique_haplotype_count
26
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 TCTCTTTCGAGCGGAAATTCA TCGAACCGAGATGCTTTAGC 42.9 50.0 57.7 58.1 984-1053 bp 1.0 77.1
rpl32-trnL-UAG_p2 TCTCTTTCGAGCGGAAATTCA TCCTAAGAGCAGCGTGTCTA 42.9 50.0 57.7 57.9 958-1027 bp 1.0 77.0
rpl32-trnL-UAG_p3 TCTCTTTCGAGCGGAAATTCA AGCAGCGTGTCTACCAATTT 42.9 45.0 57.7 57.8 951-1020 bp 1.0 76.6
rpl32-trnL-UAG_p4 TCTCTTTCGAGCGGAAATTCA CCTAAGAGCAGCGTGTCTAC 42.9 55.0 57.7 57.8 957-1026 bp 1.0 76.4
rpl32-trnL-UAG_p5 TCTCTTTCGAGCGGAAATTCA CTAAGAGCAGCGTGTCTACC 42.9 55.0 57.7 57.8 956-1025 bp 1.0 76.4