Hotspot

trnS-UGA-psbZ

LSC · 37692-38033 · 342 bp

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Pi 0.0376
MarkerSeek score 74.90
Primer available yes
Label trnS-UGA-psbZ

Feature Metadata

label_name
trnS-UGA-psbZ
feature_type
igs
region
LSC
length_bp
342
variable_sites
84
pi
0.0376
unique_haplotype_count
14
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-UGA-psbZ_p1 CAACCACTCAGCCATCTCTC CAACCATCAGGAGAGGCAAA 55.0 50.0 58.0 57.8 461-501 bp 1.0 89.3
trnS-UGA-psbZ_p2 CAACCACTCAGCCATCTCTC GAGAGGCAAATACAACGGGT 55.0 50.0 58.0 57.9 451-491 bp 0.962963 88.6
trnS-UGA-psbZ_p3 GTTTTCAAGACCGGGGCTAT CAACCATCAGGAGAGGCAAA 50.0 50.0 57.9 57.8 481-521 bp 1.0 88.3
trnS-UGA-psbZ_p4 GTTTTCAAGACCGGGGCTAT GAGAGGCAAATACAACGGGT 50.0 50.0 57.9 57.9 471-511 bp 0.962963 87.7
trnS-UGA-psbZ_p5 CGAGCAAAAGGAGAGAGAGG GAGAGGCAAATACAACGGGT 55.0 50.0 57.7 57.9 531-571 bp 0.962963 86.0