Hotspot

psbE-petL

LSC · 67961-69294 · 1334 bp

Back to Styrax

Pi 0.0086
MarkerSeek score 63.20
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1334
variable_sites
66
pi
0.0086
unique_haplotype_count
24
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 CAACCCGCAATGAATAGGGA TGAGGTTATAGTTAAAGCCGCT 50.0 40.9 57.9 57.3 1494-1552 bp 1.0 61.3
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC TGAGGTTATAGTTAAAGCCGCT 50.0 40.9 57.8 57.3 1417-1475 bp 1.0 60.6
psbE-petL_p3 ACGAATAACCAACCCGCAAT TGAGGTTATAGTTAAAGCCGCT 45.0 40.9 58.2 57.3 1503-1561 bp 1.0 60.3
psbE-petL_p4 TGCTGACGAATAACCAACCC TGAGGTTATAGTTAAAGCCGCT 50.0 40.9 58.2 57.3 1508-1566 bp 1.0 60.2
psbE-petL_p5 CGAATAACCAACCCGCAATG TGAGGTTATAGTTAAAGCCGCT 50.0 40.9 57.8 57.3 1502-1560 bp 1.0 60.2