Hotspot

rps16-trnQ-UUG

LSC · 6063-7669 · 1607 bp

Back to Styrax

Pi 0.0134
MarkerSeek score 67.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1607
variable_sites
121
pi
0.0134
unique_haplotype_count
25
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1567-1794 bp 1.0 89.0
rps16-trnQ-UUG_p2 ATGTCTTTCAAGTCGCACGT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1569-1796 bp 1.0 88.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1560-1787 bp 1.0 88.0
rps16-trnQ-UUG_p4 CATGTCTTTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 1570-1797 bp 1.0 87.3
rps16-trnQ-UUG_p5 TGTCTTTCAAGTCGCACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 1568-1795 bp 1.0 86.3