Hotspot

petN-psbM

LSC · 29782-30950 · 1169 bp

Back to Styrax

Pi 0.00851
MarkerSeek score 62.70
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1169
variable_sites
67
pi
0.00851
unique_haplotype_count
22
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 AGTAAATCTCGCTTGGGCTG AGTTCCGACTGCCTTTCTAC 50.0 50.0 58.0 57.3 1292-1344 bp 1.0 80.8
petN-psbM_p2 AGTAAATCTCGCTTGGGCTG GTTCCGACTGCCTTTCTACT 50.0 50.0 58.0 57.3 1291-1343 bp 1.0 80.8
petN-psbM_p3 AGTAAATCTCGCTTGGGCTG TCATTCTAGTTCCGACTGCC 50.0 50.0 58.0 57.0 1299-1351 bp 1.0 79.1
petN-psbM_p4 AGTAAATCTCGCTTGGGCTG CATTCTAGTTCCGACTGCCT 50.0 50.0 58.0 57.0 1298-1350 bp 1.0 79.0
petN-psbM_p5 AGTAAATCTCGCTTGGGCTG TCTAGTTCCGACTGCCTTTC 50.0 50.0 58.0 57.0 1295-1347 bp 1.0 78.5