Hotspot

psbK-psbI

LSC · 8268-8673 · 406 bp

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Pi 0.0217
MarkerSeek score 70.10
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
406
variable_sites
36
pi
0.0217
unique_haplotype_count
27
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 CAAGCTGCCGTAAGTTTTCG TCTTCACGTCCAGGATTACG 50.0 50.0 58.0 57.1 522-542 bp 1.0 81.0
psbK-psbI_p2 AGCTGCCGTAAGTTTTCGAT TCTTCACGTCCAGGATTACG 45.0 50.0 57.9 57.1 520-540 bp 1.0 80.7
psbK-psbI_p3 AAGCTGCCGTAAGTTTTCGA TCTTCACGTCCAGGATTACG 45.0 50.0 58.1 57.1 521-541 bp 1.0 80.4
psbK-psbI_p4 GCTGCCGTAAGTTTTCGATG TCTTCACGTCCAGGATTACG 50.0 50.0 57.8 57.1 519-539 bp 1.0 80.2
psbK-psbI_p5 GCAAGCTGCCGTAAGTTTTC TCTTCACGTCCAGGATTACG 50.0 50.0 58.3 57.1 523-543 bp 1.0 78.9