| Label | Region | Length (bp) | Pi | Alignment reliability | MarkerSeek score | Primer available | Action |
|---|---|---|---|---|---|---|---|
| accD | LSC | 1395 | 0.0443 | 0.90 | 78.8 | yes | View details |
| rpoA | IRb | 1074 | 0.1100 | 0.95 | 77.5 | yes | View details |
| trnN-GUU-ycf1 | SSC | 565 | 0.1039 | 0.81 | 75.9 | yes | View details |
| ycf1-trnN-GUU | SSC | 565 | 0.1106 | 0.68 | 74.4 | yes | View details |
| rpoA | IRa | 1074 | 0.1036 | 0.95 | 66.9 | no | View details |
| clpP | LSC | 1906 | 0.0475 | 0.99 | 81.8 | yes | View details |
| rpl32-trnL-UAC | SSC | 878 | 0.0346 | 1.00 | 79.7 | yes | View details |
| trnK-UUU-rps16 | LSC | 879 | 0.0313 | 0.99 | 75.9 | yes | View details |
| rbcL-accD | LSC | 779 | 0.0322 | 0.99 | 75.5 | yes | View details |
| psbH-petB | LSC | 124 | 0.0349 | 1.00 | 73.5 | yes | View details |
| ndhG-ndhI | SSC | 372 | 0.0302 | 1.00 | 72.6 | yes | View details |
| trnH-GUG-psbA | LSC | 302 | 0.0445 | 1.00 | 72.5 | yes | View details |
| rpoA-rps11 | IRb | 55 | 0.3599 | 1.00 | 72.0 | yes | View details |
| trnM-CAU-atpE | LSC | 198 | 0.0344 | 1.00 | 71.4 | yes | View details |
| trnS-GCU-trnG-UCC | LSC | 753 | 0.0251 | 0.96 | 70.6 | yes | View details |
| Candidate markers include all hotspot labels marked in the diversity plot, listed in genomic order, plus the top 10 remaining regions by MarkerSeek score. Primer design is attempted for every listed region. Primer available: yes means at least one valid primer pair was found; no means no valid pair passed screening; NA means primer design was skipped. | |||||||
Species
10
Genome length
156–165 kb
Candidate markers
289
Primer pairs
70
Genome-wide nucleotide diversity
Candidate markers
5 hotspot labels from the diversity plot in genomic order, plus the top 10 remaining regions by MarkerSeek score (out of 289 candidates).
Primer pairs
Showing the top 30 of 70 primer pairs (ranked by primer score).
| Primer ID | Label | Forward | Reverse | Amplicon (bp) | Cross-species rate | Score |
|---|---|---|---|---|---|---|
| trnH-GUG-psbA_p1 | trnH-GUG-psbA | ACTGCCTTAATCCACTTGGC |
AGGTTCGGTCAAGAGGAAGA |
671–690 | 1.000 | 88.4 |
| trnH-GUG-psbA_p2 | trnH-GUG-psbA | ACTGCCTTAATCCACTTGGC |
GAGCTTAGTTTCCGTCTGGG |
989–1008 | 1.000 | 86.1 |
| trnH-GUG-psbA_p3 | trnH-GUG-psbA | ACTGCCTTAATCCACTTGGC |
GAGTGGGAGCTTAGTTTCCG |
995–1014 | 1.000 | 86.1 |
| trnH-GUG-psbA_p4 | trnH-GUG-psbA | ACTGCCTTAATCCACTTGGC |
GGAGCTTAGTTTCCGTCTGG |
990–1009 | 1.000 | 86.1 |
| trnH-GUG-psbA_p5 | trnH-GUG-psbA | ACTGCCTTAATCCACTTGGC |
GGGAGCTTAGTTTCCGTCTG |
991–1010 | 1.000 | 86.1 |
| trnK-UUU-rps16_p1 | trnK-UUU-rps16 | AAAGCCGAGTACTCTACCGT |
GGTGCTCAACCCACAAAAAC |
963–1041 | 1.000 | 89.4 |
| trnK-UUU-rps16_p2 | trnK-UUU-rps16 | AAGCCGAGTACTCTACCGTT |
GGTGCTCAACCCACAAAAAC |
962–1040 | 1.000 | 89.4 |
| trnK-UUU-rps16_p3 | trnK-UUU-rps16 | AAGCCGAGTACTCTACCGTT |
GCCGTACGAGGAGAAAACTT |
1166–1244 | 1.000 | 88.5 |
| trnK-UUU-rps16_p4 | trnK-UUU-rps16 | AAAGCCGAGTACTCTACCGT |
AAAAGGGTATGTTGCTGCCA |
1572–1648 | 1.000 | 88.2 |
| trnK-UUU-rps16_p5 | trnK-UUU-rps16 | AAGCCGAGTACTCTACCGTT |
AAAAGGGTATGTTGCTGCCA |
1571–1647 | 1.000 | 88.2 |
| trnS-GCU-trnG-UCC_p1 | trnS-GCU-trnG-UCC | CAATCCGACGCTTTAGTCCA |
ACGAATCACACTTTTACCACT |
751–829 | 1.000 | 60.8 |
| trnS-GCU-trnG-UCC_p2 | trnS-GCU-trnG-UCC | AATCCGACGCTTTAGTCCAC |
ACGAATCACACTTTTACCACT |
750–828 | 1.000 | 60.8 |
| trnS-GCU-trnG-UCC_p3 | trnS-GCU-trnG-UCC | CAATCCGACGCTTTAGTCCA |
AACGAATCACACTTTTACCACT |
752–830 | 1.000 | 58.9 |
| trnS-GCU-trnG-UCC_p4 | trnS-GCU-trnG-UCC | AATCCGACGCTTTAGTCCAC |
AACGAATCACACTTTTACCACT |
751–829 | 1.000 | 58.9 |
| trnS-GCU-trnG-UCC_p5 | trnS-GCU-trnG-UCC | AGCAATCCGACGCTTTAGTC |
ACGAATCACACTTTTACCACT |
753–831 | 1.000 | 58.0 |
| trnM-CAU-atpE_p1 | trnM-CAU-atpE | TTTGGTTCGCTTGGGAATGA |
GGCAAATCTAGCTCTCCGAC |
356–359 | 1.000 | 84.4 |
| trnM-CAU-atpE_p2 | trnM-CAU-atpE | TTTGGTTCGCTTGGGAATGA |
GAGGCAAATCTAGCTCTCCG |
358–361 | 1.000 | 84.3 |
| trnM-CAU-atpE_p3 | trnM-CAU-atpE | GTTCCATTTGGTTCGCTTGG |
GGCAAATCTAGCTCTCCGAC |
362–365 | 1.000 | 83.9 |
| trnM-CAU-atpE_p4 | trnM-CAU-atpE | GTTCCATTTGGTTCGCTTGG |
GAGGCAAATCTAGCTCTCCG |
364–367 | 1.000 | 83.8 |
| trnM-CAU-atpE_p5 | trnM-CAU-atpE | TTTGGTTCGCTTGGGAATGA |
AGGCAAATCTAGCTCTCCGA |
357–360 | 1.000 | 83.7 |
| rbcL-accD_p1 | rbcL-accD | ACTACAGTTCGGTGGAGGAA |
AGAACCCACAAAGACGCAAT |
1309–1511 | 1.000 | 90.6 |
| rbcL-accD_p2 | rbcL-accD | ACTACAGTTCGGTGGAGGAA |
TTCTTCCGAATCGCCTCTTG |
1615–1616 | 0.200 | 48.2 |
| rbcL-accD_p3 | rbcL-accD | GGAGTCTTGAACTAGCTGCC |
TTCTTCCGAATCGCCTCTTG |
1460–1461 | 0.200 | 48.2 |
| rbcL-accD_p4 | rbcL-accD | TTGAATGCTACTGCGGGTAC |
TTCTTCCGAATCGCCTCTTG |
2094–2095 | 0.200 | 48.1 |
| rbcL-accD_p5 | rbcL-accD | ACTACAGTTCGGTGGAGGAA |
TTGGTGTGTCATATCCTGCG |
1146–1147 | 0.200 | 47.8 |
| accD_p1 | accD | GGATTGAGCCGAATGCAAAG |
ATCCAGCGCCCCTAATCTAT |
2532–2725 | 0.800 | 80.9 |
| accD_p2 | accD | AAAGGATTGAGCCGAATGCA |
ATCCAGCGCCCCTAATCTAT |
2535–2728 | 0.600 | 72.6 |
| accD_p3 | accD | AAGGATTGAGCCGAATGCAA |
ATCCAGCGCCCCTAATCTAT |
2534–2727 | 0.600 | 72.6 |
| accD_p4 | accD | AGGATTGAGCCGAATGCAAA |
ATCCAGCGCCCCTAATCTAT |
2533–2726 | 0.600 | 72.6 |
| accD_p5 | accD | AACACCGTGGAATCGATTGT |
ATCCAGCGCCCCTAATCTAT |
2025–2159 | 0.500 | 67.0 |
Result downloads
Reference species (10)
One GenBank record per species. Use the NCBI button to open the Nucleotide entry for the listed accession.
| Species | Accession | Length (bp) | Link |
|---|---|---|---|
| Amphilophium carolinae | NC_042933.1 | 163515 | View on NCBI ↗ |
| Amphilophium chocoense | NC_042914.1 | 156951 | View on NCBI ↗ |
| Amphilophium cuneifolium | NC_042915.1 | 157070 | View on NCBI ↗ |
| Amphilophium dolichoides | NC_042932.1 | 163755 | View on NCBI ↗ |
| Amphilophium dusenianum | NC_042916.1 | 163693 | View on NCBI ↗ |
| Amphilophium ecuadorense | NC_042917.1 | 163543 | View on NCBI ↗ |
| Amphilophium lactiflorum | NC_042931.1 | 155942 | View on NCBI ↗ |
| Amphilophium paniculatum | NC_042918.1 | 163710 | View on NCBI ↗ |
| Amphilophium pilosum | NC_042919.1 | 163689 | View on NCBI ↗ |
| Amphilophium steyermarkii | NC_042934.1 | 164749 | View on NCBI ↗ |