Hotspot

trnH-GUG-psbA

LSC · 92-393 · 302 bp

Back to Amphilophium

Pi 0.0445
MarkerSeek score 72.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
302
variable_sites
37
pi
0.0445
unique_haplotype_count
9
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 ACTGCCTTAATCCACTTGGC AGGTTCGGTCAAGAGGAAGA 50.0 50.0 58.2 58.0 671-690 bp 1.0 88.4
trnH-GUG-psbA_p2 ACTGCCTTAATCCACTTGGC GAGCTTAGTTTCCGTCTGGG 50.0 55.0 58.2 58.0 989-1008 bp 1.0 86.1
trnH-GUG-psbA_p3 ACTGCCTTAATCCACTTGGC GAGTGGGAGCTTAGTTTCCG 50.0 55.0 58.2 58.0 995-1014 bp 1.0 86.1
trnH-GUG-psbA_p4 ACTGCCTTAATCCACTTGGC GGAGCTTAGTTTCCGTCTGG 50.0 55.0 58.2 58.0 990-1009 bp 1.0 86.1
trnH-GUG-psbA_p5 ACTGCCTTAATCCACTTGGC GGGAGCTTAGTTTCCGTCTG 50.0 55.0 58.2 58.0 991-1010 bp 1.0 86.1