Hotspot

accD

LSC · 57114-58508 · 1395 bp

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Pi 0.0443

MarkerSeek score 78.80

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1395
variable_sites: 142
pi: 0.0443
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	GGATTGAGCCGAATGCAAAG	ATCCAGCGCCCCTAATCTAT	50.0	50.0	57.8	58.0	2532-2725 bp	0.8	80.9
accD_p2	AAAGGATTGAGCCGAATGCA	ATCCAGCGCCCCTAATCTAT	45.0	50.0	58.2	58.0	2535-2728 bp	0.6	72.6
accD_p3	AAGGATTGAGCCGAATGCAA	ATCCAGCGCCCCTAATCTAT	45.0	50.0	58.2	58.0	2534-2727 bp	0.6	72.6
accD_p4	AGGATTGAGCCGAATGCAAA	ATCCAGCGCCCCTAATCTAT	45.0	50.0	58.2	58.0	2533-2726 bp	0.6	72.6
accD_p5	AACACCGTGGAATCGATTGT	ATCCAGCGCCCCTAATCTAT	45.0	50.0	57.8	58.0	2025-2159 bp	0.5	67.0