Hotspot

trnN-GUU-ycf1

SSC · 107166-107730 · 565 bp

Back to Amphilophium

Pi 0.104

MarkerSeek score 75.90

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: SSC
length_bp: 565
variable_sites: 114
pi: 0.104
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.81
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	CTCGACGCCAGGATGTTAAA	TGCATTGGCGCATAATAGGT	50.0	45.0	58.0	58.0	1425-1509 bp	0.2	52.2
trnN-GUU-ycf1_p2	GGTGACGATACTGTAGGGGA	TGCATTGGCGCATAATAGGT	55.0	45.0	57.9	58.0	1463-1547 bp	0.2	51.7
trnN-GUU-ycf1_p3	GTGTGGGTCTTCAGCGTATT	TGCATTGGCGCATAATAGGT	50.0	45.0	57.9	58.0	1009-1093 bp	0.2	51.6
trnN-GUU-ycf1_p4	TTCTCTCCCACTTCACACCT	TGCATTGGCGCATAATAGGT	50.0	45.0	57.9	58.0	1313-1397 bp	0.2	51.4
trnN-GUU-ycf1_p5	CTCGACGCCAGGATGTTAAA	ATGAACACCATGAGCTGTCC	50.0	50.0	58.0	57.9	1401-1485 bp	0.2	51.4