Hotspot

ycf1-trnN-GUU

SSC · 132847-133411 · 565 bp

Back to Amphilophium

Pi 0.111

MarkerSeek score 74.40

Primer available yes

Label ycf1-trnN-GUU

Feature Metadata

label_name: ycf1-trnN-GUU
feature_type: igs
region: SSC
length_bp: 565
variable_sites: 112
pi: 0.111
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 74.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-trnN-GUU_p1	TGCATTGGCGCATAATAGGT	CTCGACGCCAGGATGTTAAA	45.0	50.0	58.0	58.0	1425-1509 bp	0.2	52.2
ycf1-trnN-GUU_p2	TGCATTGGCGCATAATAGGT	GGTGACGATACTGTAGGGGA	45.0	55.0	58.0	57.9	1463-1547 bp	0.2	51.7
ycf1-trnN-GUU_p3	TGCATTGGCGCATAATAGGT	GTGTGGGTCTTCAGCGTATT	45.0	50.0	58.0	57.9	1009-1093 bp	0.2	51.6
ycf1-trnN-GUU_p4	TGCATTGGCGCATAATAGGT	TTCTCTCCCACTTCACACCT	45.0	50.0	58.0	57.9	1313-1397 bp	0.2	51.4
ycf1-trnN-GUU_p5	ATGAACACCATGAGCTGTCC	CTCGACGCCAGGATGTTAAA	50.0	50.0	57.9	58.0	1401-1485 bp	0.2	51.4