Hotspot

rpl32-trnL-UAC

SSC · 116794-117671 · 878 bp

Back to Amphilophium

Pi 0.0346
MarkerSeek score 79.70
Primer available yes
Label rpl32-trnL-UAC

Feature Metadata

label_name
rpl32-trnL-UAC
feature_type
igs
region
SSC
length_bp
878
variable_sites
93
pi
0.0346
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAC_p1 TCTACCGGGAATTCAAAAAGT CTCGCACTGCTTCCTAAGAG 38.1 55.0 55.1 58.1 987-1005 bp 1.0 58.3
rpl32-trnL-UAC_p2 TCTACCGGGAATTCAAAAAGT CTCTCGCACTGCTTCCTAAG 38.1 55.0 55.1 58.1 989-1007 bp 1.0 58.2
rpl32-trnL-UAC_p3 TCTACCGGGAATTCAAAAAGT TCCTAAGAGCAGCGTGTCTA 38.1 50.0 55.1 57.9 976-994 bp 1.0 58.0
rpl32-trnL-UAC_p4 TCTACCGGGAATTCAAAAAGT AGCAGCGTGTCTACCAATTT 38.1 45.0 55.1 57.8 969-987 bp 1.0 57.6
rpl32-trnL-UAC_p5 TCTACCGGGAATTCAAAAAGT CTAAGAGCAGCGTGTCTACC 38.1 55.0 55.1 57.8 974-992 bp 1.0 57.5