Hotspot

psbH-petB

LSC · 74184-74307 · 124 bp

Back to Amphilophium

Pi 0.0349

MarkerSeek score 73.50

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 124
variable_sites: 14
pi: 0.0349
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	TCTTCCGTTTTATTGGATGGA	CATCTCTATCAAGGTGACAGACC	38.1	47.8	54.9	58.1	226-232 bp	0.9	45.0
psbH-petB_p2	TCTTCCGTTTTATTGGATGGA	ACCATCTCTATCAAGGTGACAG	38.1	45.5	54.9	57.0	228-234 bp	0.9	44.9
psbH-petB_p3	TCTTCCGTTTTATTGGATGGA	TCTCTATCAAGGTGACAGACC	38.1	47.6	54.9	56.2	224-230 bp	0.9	44.7
psbH-petB_p4	TCTTCCGTTTTATTGGATGGAA	TCTCTATCAAGGTGACAGACC	36.4	47.6	55.7	56.2	224-230 bp	0.9	44.7
psbH-petB_p5	TTCTTCCGTTTTATTGGATGGA	TCTCTATCAAGGTGACAGACC	36.4	47.6	55.7	56.2	225-231 bp	0.9	44.7