Hotspot

clpP

LSC · 69533-71438 · 1906 bp

Back to Amphilophium

Pi 0.0475

MarkerSeek score 81.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 1906
variable_sites: 269
pi: 0.0475
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCTTTGGCTCTACCCCTGAA	ACGCAATGGGGGATTGATAC	50.0	50.0	58.0	58.0	2842-2981 bp	0.8	82.7
clpP_p2	TGGAATTGGCTGTCTTGCAT	TTCGTTACAGTCCCTCCTGT	45.0	50.0	58.1	58.0	2806-2947 bp	0.8	82.2
clpP_p3	AACCTGAAATCACGGATCCG	TTCGTTACAGTCCCTCCTGT	50.0	50.0	58.0	58.0	2662-2677 bp	0.2	47.9
clpP_p4	AACCTGAAATCACGGATCCG	CCCAGCTACACCTTCGTAAC	50.0	55.0	58.0	58.0	2696-2711 bp	0.2	47.8
clpP_p5	AACCTGAAATCACGGATCCG	ACGCAATGGGGGATTGATAC	50.0	50.0	58.0	58.0	2207-2222 bp	0.2	47.8