Hotspot

trnK-UUU-rps16

LSC · 4260-5138 · 879 bp

Back to Amphilophium

Pi 0.0313
MarkerSeek score 75.90
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
879
variable_sites
85
pi
0.0313
unique_haplotype_count
10
primer_available
yes
alignment_reliability
0.99
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGTGCTCAACCCACAAAAAC 50.0 50.0 58.2 58.1 963-1041 bp 1.0 89.4
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGTGCTCAACCCACAAAAAC 50.0 50.0 58.2 58.1 962-1040 bp 1.0 89.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GCCGTACGAGGAGAAAACTT 50.0 50.0 58.2 57.9 1166-1244 bp 1.0 88.5
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.2 58.0 1572-1648 bp 1.0 88.2
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT AAAAGGGTATGTTGCTGCCA 50.0 45.0 58.2 58.0 1571-1647 bp 1.0 88.2