primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	ACTGCCTTAATCCACTTGGC	AGGTTCGGTCAAGAGGAAGA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.171699	92	393	671-690	1.000000	88.4
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	ACTGCCTTAATCCACTTGGC	GAGCTTAGTTTCCGTCTGGG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.172116	92	393	989-1008	1.000000	86.1
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	ACTGCCTTAATCCACTTGGC	GAGTGGGAGCTTAGTTTCCG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.172116	92	393	995-1014	1.000000	86.1
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACTGCCTTAATCCACTTGGC	GGAGCTTAGTTTCCGTCTGG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.172116	92	393	990-1009	1.000000	86.1
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	ACTGCCTTAATCCACTTGGC	GGGAGCTTAGTTTCCGTCTG	20	20	50.0	55.0	58.2	58.0	0.0	0.0	0.172116	92	393	991-1010	1.000000	86.1
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	GGTGCTCAACCCACAAAAAC	20	20	50.0	50.0	58.2	58.1	20.3	0.0	0.233831	4260	5138	963-1041	1.000000	89.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	GGTGCTCAACCCACAAAAAC	20	20	50.0	50.0	58.2	58.1	20.3	0.0	0.233831	4260	5138	962-1040	1.000000	89.4
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAGCCGAGTACTCTACCGTT	GCCGTACGAGGAGAAAACTT	20	20	50.0	50.0	58.2	57.9	20.3	18.8	0.244399	4260	5138	1166-1244	1.000000	88.5
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAAGCCGAGTACTCTACCGT	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.2	58.0	20.3	0.0	0.176662	4260	5138	1572-1648	1.000000	88.2
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	AAGCCGAGTACTCTACCGTT	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.2	58.0	20.3	0.0	0.176662	4260	5138	1571-1647	1.000000	88.2
trnS-GCU-trnG-UCC_p1	trnS-GCU-trnG-UCC	1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.540425	8013	8765	751-829	1.000000	60.8
trnS-GCU-trnG-UCC_p2	trnS-GCU-trnG-UCC	2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.0	55.5	0.0	0.0	3.541813	8013	8765	750-828	1.000000	60.8
trnS-GCU-trnG-UCC_p3	trnS-GCU-trnG-UCC	3	CAATCCGACGCTTTAGTCCA	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.0	56.2	0.0	0.0	3.780896	8013	8765	752-830	1.000000	58.9
trnS-GCU-trnG-UCC_p4	trnS-GCU-trnG-UCC	4	AATCCGACGCTTTAGTCCAC	AACGAATCACACTTTTACCACT	20	22	50.0	36.4	58.0	56.2	0.0	0.0	3.782284	8013	8765	751-829	1.000000	58.9
trnS-GCU-trnG-UCC_p5	trnS-GCU-trnG-UCC	5	AGCAATCCGACGCTTTAGTC	ACGAATCACACTTTTACCACT	20	21	50.0	38.1	58.4	55.5	0.0	0.0	3.894265	8013	8765	753-831	1.000000	58.0
trnM-CAU-atpE_p1	trnM-CAU-atpE	1	TTTGGTTCGCTTGGGAATGA	GGCAAATCTAGCTCTCCGAC	20	20	45.0	55.0	58.0	58.1	3.6	0.0	0.152324	52027	52224	356-359	1.000000	84.4
trnM-CAU-atpE_p2	trnM-CAU-atpE	2	TTTGGTTCGCTTGGGAATGA	GAGGCAAATCTAGCTCTCCG	20	20	45.0	55.0	58.0	57.9	3.6	0.0	0.159142	52027	52224	358-361	1.000000	84.3
trnM-CAU-atpE_p3	trnM-CAU-atpE	3	GTTCCATTTGGTTCGCTTGG	GGCAAATCTAGCTCTCCGAC	20	20	50.0	55.0	57.9	58.1	0.0	0.0	0.205190	52027	52224	362-365	1.000000	83.9
trnM-CAU-atpE_p4	trnM-CAU-atpE	4	GTTCCATTTGGTTCGCTTGG	GAGGCAAATCTAGCTCTCCG	20	20	50.0	55.0	57.9	57.9	0.0	0.0	0.212009	52027	52224	364-367	1.000000	83.8
trnM-CAU-atpE_p5	trnM-CAU-atpE	5	TTTGGTTCGCTTGGGAATGA	AGGCAAATCTAGCTCTCCGA	20	20	45.0	50.0	58.0	58.2	3.6	0.0	0.229920	52027	52224	357-360	1.000000	83.7
rbcL-accD_p1	rbcL-accD	1	ACTACAGTTCGGTGGAGGAA	AGAACCCACAAAGACGCAAT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.024114	56335	57113	1309-1511	1.000000	90.6
rbcL-accD_p2	rbcL-accD	2	ACTACAGTTCGGTGGAGGAA	TTCTTCCGAATCGCCTCTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.015515	56335	57113	1615-1616	0.200000	48.2
rbcL-accD_p3	rbcL-accD	3	GGAGTCTTGAACTAGCTGCC	TTCTTCCGAATCGCCTCTTG	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.027283	56335	57113	1460-1461	0.200000	48.2
rbcL-accD_p4	rbcL-accD	4	TTGAATGCTACTGCGGGTAC	TTCTTCCGAATCGCCTCTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028687	56335	57113	2094-2095	0.200000	48.1
rbcL-accD_p5	rbcL-accD	5	ACTACAGTTCGGTGGAGGAA	TTGGTGTGTCATATCCTGCG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.028135	56335	57113	1146-1147	0.200000	47.8
accD_p1	accD	1	GGATTGAGCCGAATGCAAAG	ATCCAGCGCCCCTAATCTAT	20	20	50.0	50.0	57.8	58.0	0.0	2.3	0.241003	57114	58508	2532-2725	0.800000	80.9
accD_p2	accD	2	AAAGGATTGAGCCGAATGCA	ATCCAGCGCCCCTAATCTAT	20	20	45.0	50.0	58.2	58.0	0.0	2.3	0.203600	57114	58508	2535-2728	0.600000	72.6
accD_p3	accD	3	AAGGATTGAGCCGAATGCAA	ATCCAGCGCCCCTAATCTAT	20	20	45.0	50.0	58.2	58.0	0.0	2.3	0.203600	57114	58508	2534-2727	0.600000	72.6
accD_p4	accD	4	AGGATTGAGCCGAATGCAAA	ATCCAGCGCCCCTAATCTAT	20	20	45.0	50.0	58.2	58.0	0.0	2.3	0.203600	57114	58508	2533-2726	0.600000	72.6
accD_p5	accD	5	AACACCGTGGAATCGATTGT	ATCCAGCGCCCCTAATCTAT	20	20	45.0	50.0	57.8	58.0	17.0	2.3	0.220624	57114	58508	2025-2159	0.500000	67.0
clpP_p1	clpP	1	TCTTTGGCTCTACCCCTGAA	ACGCAATGGGGGATTGATAC	20	20	50.0	50.0	58.0	58.0	0.0	1.6	0.057606	69533	71438	2842-2981	0.800000	82.7
clpP_p2	clpP	2	TGGAATTGGCTGTCTTGCAT	TTCGTTACAGTCCCTCCTGT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.077883	69533	71438	2806-2947	0.800000	82.2
clpP_p3	clpP	3	AACCTGAAATCACGGATCCG	TTCGTTACAGTCCCTCCTGT	20	20	50.0	50.0	58.0	58.0	32.2	0.0	0.029546	69533	71438	2662-2677	0.200000	47.9
clpP_p4	clpP	4	AACCTGAAATCACGGATCCG	CCCAGCTACACCTTCGTAAC	20	20	50.0	55.0	58.0	58.0	32.2	0.0	0.030146	69533	71438	2696-2711	0.200000	47.8
clpP_p5	clpP	5	AACCTGAAATCACGGATCCG	ACGCAATGGGGGATTGATAC	20	20	50.0	50.0	58.0	58.0	32.2	1.6	0.042919	69533	71438	2207-2222	0.200000	47.8
psbH-petB_p1	psbH-petB	1	TCTTCCGTTTTATTGGATGGA	CATCTCTATCAAGGTGACAGACC	21	23	38.1	47.8	54.9	58.1	0.0	0.0	7.144303	74184	74307	226-232	0.900000	45.0
psbH-petB_p2	psbH-petB	2	TCTTCCGTTTTATTGGATGGA	ACCATCTCTATCAAGGTGACAG	21	22	38.1	45.5	54.9	57.0	0.0	0.0	7.104776	74184	74307	228-234	0.900000	44.9
psbH-petB_p3	psbH-petB	3	TCTTCCGTTTTATTGGATGGA	TCTCTATCAAGGTGACAGACC	21	21	38.1	47.6	54.9	56.2	0.0	0.0	6.893869	74184	74307	224-230	0.900000	44.7
psbH-petB_p4	psbH-petB	4	TCTTCCGTTTTATTGGATGGAA	TCTCTATCAAGGTGACAGACC	22	21	36.4	47.6	55.7	56.2	0.0	0.0	7.099375	74184	74307	224-230	0.900000	44.7
psbH-petB_p5	psbH-petB	5	TTCTTCCGTTTTATTGGATGGA	TCTCTATCAAGGTGACAGACC	22	21	36.4	47.6	55.7	56.2	0.0	0.0	7.099375	74184	74307	225-231	0.900000	44.7
rpoA_p1	rpoA	1	GGGCTTAGCGGTTCTAGAAC	CCGAGAACTGGTTCACGTAG	20	20	55.0	55.0	58.1	58.0	24.7	23.4	0.084180	77315	78388	2018-2191	1.000000	91.3
rpoA_p2	rpoA	2	GGGCTTAGCGGTTCTAGAAC	GGAAGAGACGCAGCATTACA	20	20	55.0	50.0	58.1	58.0	24.7	0.0	0.072867	77315	78388	1709-1858	0.600000	75.4
rpoA_p3	rpoA	3	TTGGTTGGGTATTGGTGCAA	GGAAGAGACGCAGCATTACA	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.098249	77315	78388	1442-1591	0.600000	75.2
rpoA_p4	rpoA	4	GGGCTTAGCGGTTCTAGAAC	ATTCGCCGAAGTCGTATACG	20	20	55.0	50.0	58.1	58.0	24.7	19.2	0.091009	77315	78388	1776-1781	0.200000	48.7
rpoA_p5	rpoA	5	GGGCTTAGCGGTTCTAGAAC	GCAACGAGCAGGAGTTATGA	20	20	55.0	50.0	58.1	58.0	24.7	0.0	0.072867	77315	78388	1837-1837	0.100000	43.4
rpoA-rps11_p1	rpoA-rps11	1	CAAGTGCTTCTACCGGAGTC	CCGAGAACTGGTTCACGTAG	20	20	55.0	55.0	58.0	58.0	9.5	23.4	0.023789	78389	78443	1137-1304	1.000000	91.8
rpoA-rps11_p2	rpoA-rps11	2	CAAGTGCTTCTACCGGAGTC	GGAAGAGACGCAGCATTACA	20	20	55.0	50.0	58.0	58.0	9.5	0.0	0.012476	78389	78443	828-971	0.600000	75.9
rpoA-rps11_p3	rpoA-rps11	3	CAAGTGCTTCTACCGGAGTC	ATTCGCCGAAGTCGTATACG	20	20	55.0	50.0	58.0	58.0	9.5	19.2	0.030618	78389	78443	866-866	0.200000	50.2
rpoA-rps11_p4	rpoA-rps11	4	AACGCATCTCCACTGTGTTT	GGAAGAGACGCAGCATTACA	20	20	45.0	50.0	58.0	58.0	7.1	0.0	0.042826	78389	78443	226-226	0.200000	49.1
rpoA-rps11_p5	rpoA-rps11	5	CAAGTGCTTCTACCGGAGTC	GCAACGAGCAGGAGTTATGA	20	20	55.0	50.0	58.0	58.0	9.5	0.0	0.012476	78389	78443	927-927	0.100000	43.9
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	CTCGACGCCAGGATGTTAAA	TGCATTGGCGCATAATAGGT	20	20	50.0	45.0	58.0	58.0	0.0	10.8	0.024728	107166	107730	1425-1509	0.200000	52.2
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	GGTGACGATACTGTAGGGGA	TGCATTGGCGCATAATAGGT	20	20	55.0	45.0	57.9	58.0	0.0	10.8	0.083116	107166	107730	1463-1547	0.200000	51.7
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GTGTGGGTCTTCAGCGTATT	TGCATTGGCGCATAATAGGT	20	20	50.0	45.0	57.9	58.0	0.0	10.8	0.114908	107166	107730	1009-1093	0.200000	51.6
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	TTCTCTCCCACTTCACACCT	TGCATTGGCGCATAATAGGT	20	20	50.0	45.0	57.9	58.0	0.0	10.8	0.123299	107166	107730	1313-1397	0.200000	51.4
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	CTCGACGCCAGGATGTTAAA	ATGAACACCATGAGCTGTCC	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.124384	107166	107730	1401-1485	0.200000	51.4
rpl32-trnL-UAC_p1	rpl32-trnL-UAC	1	TCTACCGGGAATTCAAAAAGT	CTCGCACTGCTTCCTAAGAG	21	20	38.1	55.0	55.1	58.1	6.3	0.0	3.955244	116794	117671	987-1005	1.000000	58.3
rpl32-trnL-UAC_p2	rpl32-trnL-UAC	2	TCTACCGGGAATTCAAAAAGT	CTCTCGCACTGCTTCCTAAG	21	20	38.1	55.0	55.1	58.1	6.3	0.0	3.955244	116794	117671	989-1007	1.000000	58.2
rpl32-trnL-UAC_p3	rpl32-trnL-UAC	3	TCTACCGGGAATTCAAAAAGT	TCCTAAGAGCAGCGTGTCTA	21	20	38.1	50.0	55.1	57.9	6.3	0.0	3.999439	116794	117671	976-994	1.000000	58.0
rpl32-trnL-UAC_p4	rpl32-trnL-UAC	4	TCTACCGGGAATTCAAAAAGT	AGCAGCGTGTCTACCAATTT	21	20	38.1	45.0	55.1	57.8	6.3	0.0	4.060166	116794	117671	969-987	1.000000	57.6
rpl32-trnL-UAC_p5	rpl32-trnL-UAC	5	TCTACCGGGAATTCAAAAAGT	CTAAGAGCAGCGTGTCTACC	21	20	38.1	55.0	55.1	57.8	6.3	0.0	4.071457	116794	117671	974-992	1.000000	57.5
ndhG-ndhI_p1	ndhG-ndhI	1	AAGACCCGATCCCAGAAAGA	CAATTTCTTTGAATTCACCTCA	20	22	50.0	31.8	58.0	53.2	0.0	7.9	6.831059	122192	122563	432-472	1.000000	49.9
ndhG-ndhI_p2	ndhG-ndhI	2	AAGACCCGATCCCAGAAAGA	CAATTTCTTTGAATTCACCTCAA	20	23	50.0	30.4	58.0	54.0	0.0	7.9	7.008538	122192	122563	432-472	1.000000	49.9
ndhG-ndhI_p3	ndhG-ndhI	3	AAGACCCGATCCCAGAAAGA	CAATTTCTTTGAATTCACCTCAAA	20	24	50.0	29.2	58.0	54.8	0.0	7.9	7.253832	122192	122563	432-472	1.000000	49.9
ndhG-ndhI_p4	ndhG-ndhI	4	ACCCGATCCCAGAAAGACTA	CAATTTCTTTGAATTCACCTCA	20	22	50.0	31.8	57.5	53.2	0.0	7.9	7.328525	122192	122563	429-469	1.000000	49.9
ndhG-ndhI_p5	ndhG-ndhI	5	ACCCGATCCCAGAAAGACTA	CAATTTCTTTGAATTCACCTCAA	20	23	50.0	30.4	57.5	54.0	0.0	7.9	7.506003	122192	122563	429-469	1.000000	49.9
ycf1-trnN-GUU_p1	ycf1-trnN-GUU	1	TGCATTGGCGCATAATAGGT	CTCGACGCCAGGATGTTAAA	20	20	45.0	50.0	58.0	58.0	10.8	0.0	0.024728	132847	133411	1425-1509	0.200000	52.2
ycf1-trnN-GUU_p2	ycf1-trnN-GUU	2	TGCATTGGCGCATAATAGGT	GGTGACGATACTGTAGGGGA	20	20	45.0	55.0	58.0	57.9	10.8	0.0	0.083116	132847	133411	1463-1547	0.200000	51.7
ycf1-trnN-GUU_p3	ycf1-trnN-GUU	3	TGCATTGGCGCATAATAGGT	GTGTGGGTCTTCAGCGTATT	20	20	45.0	50.0	58.0	57.9	10.8	0.0	0.114908	132847	133411	1009-1093	0.200000	51.6
ycf1-trnN-GUU_p4	ycf1-trnN-GUU	4	TGCATTGGCGCATAATAGGT	TTCTCTCCCACTTCACACCT	20	20	45.0	50.0	58.0	57.9	10.8	0.0	0.123299	132847	133411	1313-1397	0.200000	51.4
ycf1-trnN-GUU_p5	ycf1-trnN-GUU	5	ATGAACACCATGAGCTGTCC	CTCGACGCCAGGATGTTAAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.124384	132847	133411	1401-1485	0.200000	51.4