| Label | Region | Length (bp) | Pi | Alignment reliability | MarkerSeek score | Primer available | Action |
|---|---|---|---|---|---|---|---|
| accD | Genome | 2211 | 0.0181 | 1.00 | 66.2 | yes | View details |
| accD-infA | Genome | 1880 | 0.0336 | 0.92 | 72.6 | yes | View details |
| ycf2 | Genome | 6810 | 0.0148 | 0.99 | 63.1 | yes | View details |
| CD863_pgr004-CD863_pgr003 | Genome | 2240 | 0.0503 | 0.81 | 83.5 | yes | View details |
| ycf1-chlN | Genome | 1084 | 0.0356 | 0.85 | 73.7 | yes | View details |
| atpF-atpA | Genome | 93 | 0.0354 | 1.00 | 82.5 | yes | View details |
| CD863_pgr002-CD863_pgr001 | Genome | 215 | 0.0258 | 1.00 | 78.3 | yes | View details |
| trnV-GAC-CD863_pgr004 | Genome | 497 | 0.0232 | 0.97 | 78.0 | yes | View details |
| psbE-petL | Genome | 1275 | 0.0109 | 0.99 | 74.3 | yes | View details |
| atpE-rbcL | Genome | 508 | 0.0137 | 0.98 | 73.9 | yes | View details |
| chlL-trnH-GUG | Genome | 207 | 0.0490 | 0.95 | 73.7 | yes | View details |
| trnV-UAC | Genome | 37 | 0.0305 | 1.00 | 70.3 | yes | View details |
| ndhF-rpl32 | Genome | 811 | 0.0059 | 1.00 | 69.8 | yes | View details |
| psbD-trnT-GGU | Genome | 1138 | 0.0045 | 1.00 | 69.0 | yes | View details |
| psbI-psbK | Genome | 644 | 0.0137 | 1.00 | 68.5 | yes | View details |
| Candidate markers include all hotspot labels marked in the diversity plot, listed in genomic order, plus the top 10 remaining regions by MarkerSeek score. Primer design is attempted for every listed region. Primer available: yes means at least one valid primer pair was found; no means no valid pair passed screening; NA means primer design was skipped. | |||||||
Species
11
Genome length
127–129 kb
Candidate markers
230
Primer pairs
75
Genome-wide nucleotide diversity
Candidate markers
5 hotspot labels from the diversity plot in genomic order, plus the top 10 remaining regions by MarkerSeek score (out of 230 candidates).
Primer pairs
Showing the top 30 of 75 primer pairs (ranked by primer score).
| Primer ID | Label | Forward | Reverse | Amplicon (bp) | Cross-species rate | Score |
|---|---|---|---|---|---|---|
| psbD-trnT-GGU_p1 | psbD-trnT-GGU | ATAGACCAGACCAACCCACA |
GTGGTAGAGTAACGCCATGG |
1280–1470 | 1.000 | 84.5 |
| psbD-trnT-GGU_p2 | psbD-trnT-GGU | ATAGACCAGACCAACCCACA |
TCAGTGGTAGAGTAACGCCA |
1283–1473 | 1.000 | 84.3 |
| psbD-trnT-GGU_p3 | psbD-trnT-GGU | TAGACCAGACCAACCCACAA |
GTGGTAGAGTAACGCCATGG |
1279–1469 | 1.000 | 83.2 |
| psbD-trnT-GGU_p4 | psbD-trnT-GGU | TAGACCAGACCAACCCACAA |
TCAGTGGTAGAGTAACGCCA |
1282–1472 | 1.000 | 83.1 |
| psbD-trnT-GGU_p5 | psbD-trnT-GGU | ACAAAACGGTCTCTGCGTAG |
GTGGTAGAGTAACGCCATGG |
1257–1447 | 1.000 | 83.0 |
| atpF-atpA_p1 | atpF-atpA | GCCTGCTTAGAGCCATGAAA |
AGCAATGCCATCTCCTACTTG |
222–268 | 1.000 | 77.3 |
| atpF-atpA_p2 | atpF-atpA | GCCTGCTTAGAGCCATGAAA |
GCAATGCCATCTCCTACTTGA |
221–267 | 1.000 | 77.3 |
| atpF-atpA_p3 | atpF-atpA | AATATCGGCCTGCTTAGAGC |
AGCAATGCCATCTCCTACTTG |
229–275 | 1.000 | 75.5 |
| atpF-atpA_p4 | atpF-atpA | AATATCGGCCTGCTTAGAGC |
GCAATGCCATCTCCTACTTGA |
228–274 | 1.000 | 75.5 |
| atpF-atpA_p5 | atpF-atpA | GCCTGCTTAGAGCCATGAAA |
AGCAATGCCATCTCCTACTT |
222–268 | 1.000 | 74.6 |
| psbI-psbK_p1 | psbI-psbK | TCTTTACGCCCAGGATTTCG |
CAAGCTGCCGTAAGTTTTCG |
751–790 | 1.000 | 84.7 |
| psbI-psbK_p2 | psbI-psbK | TCGTCCTGGATCGTTCGATA |
CAAGCTGCCGTAAGTTTTCG |
734–773 | 1.000 | 84.6 |
| psbI-psbK_p3 | psbI-psbK | TCTTTACGCCCAGGATTTCG |
AGCTGCCGTAAGTTTTCGAT |
749–788 | 1.000 | 84.4 |
| psbI-psbK_p4 | psbI-psbK | ATTTCGTCCTGGATCGTTCG |
CAAGCTGCCGTAAGTTTTCG |
737–776 | 1.000 | 84.3 |
| psbI-psbK_p5 | psbI-psbK | TCTTTACGCCCAGGATTTCG |
GCCCGAGGCTTATGCTATTT |
836–875 | 1.000 | 83.8 |
| trnV-UAC_p1 | trnV-UAC | AGGCGGAATATAAGAGTTTCC |
TGCATGTTGGGTTCTTGGAA |
119–132 | 1.000 | 56.5 |
| trnV-UAC_p2 | trnV-UAC | AGGCGGAATATAAGAGTTTCC |
AATGAAAGAGCCCAATGCCA |
145–158 | 1.000 | 56.0 |
| trnV-UAC_p3 | trnV-UAC | AGAGACAATAAGAAATAGGCGGA |
TGCATGTTGGGTTCTTGGAA |
135–148 | 1.000 | 55.8 |
| trnV-UAC_p4 | trnV-UAC | AGAGACAATAAGAAATAGGCGGA |
AATGAAAGAGCCCAATGCCA |
161–174 | 1.000 | 55.2 |
| trnV-UAC_p5 | trnV-UAC | AGGCGGAATATAAGAGTTTCC |
ATGAAAGAGCCCAATGCCAT |
144–157 | 1.000 | 55.2 |
| atpE-rbcL_p1 | atpE-rbcL | AGAACACGACTAGAGGCTGT |
GCTTTGAATCCGACACTTGC |
528–603 | 1.000 | 86.2 |
| atpE-rbcL_p2 | atpE-rbcL | ATCTTGGACGAGTTGAAGGC |
GCTTTGAATCCGACACTTGC |
590–665 | 1.000 | 85.9 |
| atpE-rbcL_p3 | atpE-rbcL | GGACGAGTTGAAGGCAAGAA |
GCTTTGAATCCGACACTTGC |
585–660 | 1.000 | 85.8 |
| atpE-rbcL_p4 | atpE-rbcL | AGAACACGACTAGAGGCTGT |
CCAGCTTTGAATCCGACACT |
531–606 | 1.000 | 84.9 |
| atpE-rbcL_p5 | atpE-rbcL | ATCTTGGACGAGTTGAAGGC |
CCAGCTTTGAATCCGACACT |
593–668 | 1.000 | 84.6 |
| accD_p1 | accD | ACGATGAAATAAAGGAGACAAGT |
TCTGATATTAGTTGTGCGCCT |
2298–2412 | 1.000 | 48.2 |
| accD_p2 | accD | ACGATGAAATAAAGGAGACAAGT |
GTCTGATATTAGTTGTGCGCC |
2299–2413 | 1.000 | 48.2 |
| accD_p3 | accD | AACGATGAAATAAAGGAGACAAGT |
TCTGATATTAGTTGTGCGCCT |
2299–2413 | 1.000 | 48.2 |
| accD_p4 | accD | AACGATGAAATAAAGGAGACAAGT |
GTCTGATATTAGTTGTGCGCC |
2300–2414 | 1.000 | 48.2 |
| accD_p5 | accD | ACGATGAAATAAAGGAGACAAGT |
TCTGATATTAGTTGTGCGCC |
2298–2412 | 1.000 | 48.2 |
Result downloads
Reference species (11)
One GenBank record per species. Use the NCBI button to open the Nucleotide entry for the listed accession.
| Species | Accession | Length (bp) | Link |
|---|---|---|---|
| Taxus baccata | NC_035066.1 | 128653 | View on NCBI ↗ |
| Taxus calcicola | NC_041501.1 | 128270 | View on NCBI ↗ |
| Taxus canadensis | NC_041499.1 | 128286 | View on NCBI ↗ |
| Taxus chinensis | NC_041496.1 | 128444 | View on NCBI ↗ |
| Taxus contorta | NC_041497.1 | 127806 | View on NCBI ↗ |
| Taxus cuspidata | NC_041498.1 | 128109 | View on NCBI ↗ |
| Taxus florinii | NC_041504.1 | 127335 | View on NCBI ↗ |
| Taxus mairei | NC_020321.1 | 127665 | View on NCBI ↗ |
| Taxus phytonii | NC_041495.1 | 128361 | View on NCBI ↗ |
| Taxus wallichiana | NC_041466.1 | 128276 | View on NCBI ↗ |
| Taxus wallichiana var. yunnanensis | NC_073116.1 | 128141 | View on NCBI ↗ |