Hotspot

psbE-petL

Genome · 69281-70555 · 1275 bp

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Pi 0.0109
MarkerSeek score 74.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
Genome
length_bp
1275
variable_sites
51
pi
0.0109
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 TTCTCCCGTATTCCCAGACA AGGACTAAGGTTAAAATAAGCAGA 50.0 33.3 57.8 55.5 1296-1380 bp 1.0 47.2
psbE-petL_p2 TTCTCCCGTATTCCCAGACA GGACTAAGGTTAAAATAAGCAGAACT 50.0 34.6 57.8 57.4 1295-1379 bp 1.0 47.2
psbE-petL_p3 ATAATGTCAGCAAAAGCGCG AGGACTAAGGTTAAAATAAGCAGA 45.0 33.3 57.8 55.5 1316-1400 bp 1.0 47.1
psbE-petL_p4 ATAATGTCAGCAAAAGCGCG AGGACTAAGGTTAAAATAAGCAGAAC 45.0 34.6 57.8 57.4 1316-1400 bp 1.0 47.1
psbE-petL_p5 ATAATGTCAGCAAAAGCGCG GGACTAAGGTTAAAATAAGCAGAACT 45.0 34.6 57.8 57.4 1315-1399 bp 1.0 47.1