Hotspot

CD863_pgr004-CD863_pgr003

Genome · 96702-98941 · 2240 bp

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Pi 0.0503
MarkerSeek score 83.50
Primer available yes
Label CD863_pgr004-CD863_pgr003

Feature Metadata

label_name
CD863_pgr004-CD863_pgr003
feature_type
igs
region
Genome
length_bp
2240
variable_sites
238
pi
0.0503
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.81
markerseek_score
83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
CD863_pgr004-CD863_pgr003_p1 GACCGGAGTGAAGTCGTAAC GTCTCTGGGTGCCTAGGTAT 55.0 55.0 58.0 57.9 1748-2348 bp 1.0 91.1
CD863_pgr004-CD863_pgr003_p2 GACCGGAGTGAAGTCGTAAC ATGCTCAATTTCCACTCCCC 55.0 50.0 58.0 57.9 1800-2400 bp 1.0 90.6
CD863_pgr004-CD863_pgr003_p3 GACCGGAGTGAAGTCGTAAC CACCGTAAGCCCTTCCATTT 55.0 50.0 58.0 58.2 1727-2327 bp 1.0 90.5
CD863_pgr004-CD863_pgr003_p4 GACCGGAGTGAAGTCGTAAC AAGCATTTCGTCGCTTACCA 55.0 45.0 58.0 58.2 1779-2379 bp 1.0 90.2
CD863_pgr004-CD863_pgr003_p5 GACCGGAGTGAAGTCGTAAC GTATCCACCGTAAGCCCTTC 55.0 55.0 58.0 57.8 1732-2332 bp 1.0 89.9