Hotspot

atpE-rbcL

Genome · 50094-50601 · 508 bp

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Pi 0.0137
MarkerSeek score 73.90
Primer available yes
Label atpE-rbcL

Feature Metadata

label_name
atpE-rbcL
feature_type
igs
region
Genome
length_bp
508
variable_sites
23
pi
0.0137
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.98
markerseek_score
73.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpE-rbcL_p1 AGAACACGACTAGAGGCTGT GCTTTGAATCCGACACTTGC 50.0 50.0 58.1 58.0 528-603 bp 1.0 86.2
atpE-rbcL_p2 ATCTTGGACGAGTTGAAGGC GCTTTGAATCCGACACTTGC 50.0 50.0 57.9 58.0 590-665 bp 1.0 85.9
atpE-rbcL_p3 GGACGAGTTGAAGGCAAGAA GCTTTGAATCCGACACTTGC 50.0 50.0 58.1 58.0 585-660 bp 1.0 85.8
atpE-rbcL_p4 AGAACACGACTAGAGGCTGT CCAGCTTTGAATCCGACACT 50.0 50.0 58.1 58.2 531-606 bp 1.0 84.9
atpE-rbcL_p5 ATCTTGGACGAGTTGAAGGC CCAGCTTTGAATCCGACACT 50.0 50.0 57.9 58.2 593-668 bp 1.0 84.6