Hotspot

trnV-UAC

Genome · 47161-47197 · 37 bp

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Pi 0.0305
MarkerSeek score 70.30
Primer available yes
Label trnV-UAC

Feature Metadata

label_name
trnV-UAC
feature_type
tRNA
region
Genome
length_bp
37
variable_sites
4
pi
0.0305
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC_p1 AGGCGGAATATAAGAGTTTCC TGCATGTTGGGTTCTTGGAA 42.9 45.0 55.2 57.9 119-132 bp 1.0 56.5
trnV-UAC_p2 AGGCGGAATATAAGAGTTTCC AATGAAAGAGCCCAATGCCA 42.9 45.0 55.2 58.1 145-158 bp 1.0 56.0
trnV-UAC_p3 AGAGACAATAAGAAATAGGCGGA TGCATGTTGGGTTCTTGGAA 39.1 45.0 57.1 57.9 135-148 bp 1.0 55.8
trnV-UAC_p4 AGAGACAATAAGAAATAGGCGGA AATGAAAGAGCCCAATGCCA 39.1 45.0 57.1 58.1 161-174 bp 1.0 55.2
trnV-UAC_p5 AGGCGGAATATAAGAGTTTCC ATGAAAGAGCCCAATGCCAT 42.9 45.0 55.2 57.8 144-157 bp 1.0 55.2