Hotspot

psbI-psbK

Genome · 41708-42351 · 644 bp

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Pi 0.0137
MarkerSeek score 68.50
Primer available yes
Label psbI-psbK

Feature Metadata

label_name
psbI-psbK
feature_type
igs
region
Genome
length_bp
644
variable_sites
27
pi
0.0137
unique_haplotype_count
10
primer_available
yes
alignment_reliability
1.00
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-psbK_p1 TCTTTACGCCCAGGATTTCG CAAGCTGCCGTAAGTTTTCG 50.0 50.0 58.0 58.0 751-790 bp 1.0 84.7
psbI-psbK_p2 TCGTCCTGGATCGTTCGATA CAAGCTGCCGTAAGTTTTCG 50.0 50.0 58.0 58.0 734-773 bp 1.0 84.6
psbI-psbK_p3 TCTTTACGCCCAGGATTTCG AGCTGCCGTAAGTTTTCGAT 50.0 45.0 58.0 57.9 749-788 bp 1.0 84.4
psbI-psbK_p4 ATTTCGTCCTGGATCGTTCG CAAGCTGCCGTAAGTTTTCG 50.0 50.0 58.1 58.0 737-776 bp 1.0 84.3
psbI-psbK_p5 TCTTTACGCCCAGGATTTCG GCCCGAGGCTTATGCTATTT 50.0 50.0 58.0 58.1 836-875 bp 1.0 83.8