Hotspot

accD

Genome · 52500-54710 · 2211 bp

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Pi 0.0181
MarkerSeek score 66.20
Primer available yes
Label accD

Feature Metadata

label_name
accD
feature_type
gene
region
Genome
length_bp
2211
variable_sites
120
pi
0.0181
unique_haplotype_count
11
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD_p1 ACGATGAAATAAAGGAGACAAGT TCTGATATTAGTTGTGCGCCT 34.8 42.9 55.9 57.2 2298-2412 bp 1.0 48.2
accD_p2 ACGATGAAATAAAGGAGACAAGT GTCTGATATTAGTTGTGCGCC 34.8 47.6 55.9 57.1 2299-2413 bp 1.0 48.2
accD_p3 AACGATGAAATAAAGGAGACAAGT TCTGATATTAGTTGTGCGCCT 33.3 42.9 56.6 57.2 2299-2413 bp 1.0 48.2
accD_p4 AACGATGAAATAAAGGAGACAAGT GTCTGATATTAGTTGTGCGCC 33.3 47.6 56.6 57.1 2300-2414 bp 1.0 48.2
accD_p5 ACGATGAAATAAAGGAGACAAGT TCTGATATTAGTTGTGCGCC 34.8 45.0 55.9 55.6 2298-2412 bp 1.0 48.2