Hotspot

ycf2

Genome · 82204-89013 · 6810 bp

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Pi 0.0148
MarkerSeek score 63.10
Primer available yes
Label ycf2

Feature Metadata

label_name
ycf2
feature_type
gene
region
Genome
length_bp
6810
variable_sites
330
pi
0.0148
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf2_p1 CTCCTTCCGCATGTGCTTAT GATTCGATATCCCTCGGTGC 50.0 55.0 58.0 58.0 2264-2612 bp 1.0 88.7
ycf2_p2 TCATCAGATCCCGGATCGAA GATTCGATATCCCTCGGTGC 50.0 55.0 58.0 58.0 1661-2015 bp 0.818182 81.9
ycf2_p3 TTCATCAGATCCCGGATCGA GATTCGATATCCCTCGGTGC 50.0 55.0 58.0 58.0 1662-2016 bp 0.818182 81.9
ycf2_p4 TCATCAGATCCCGGATCGAA GCAAAATGGGTCGATGGAATC 50.0 47.6 58.0 57.9 1021-1468 bp 0.636364 65.7
ycf2_p5 TTCATCAGATCCCGGATCGA GCAAAATGGGTCGATGGAATC 50.0 47.6 58.0 57.9 1022-1469 bp 0.636364 65.7