| Label | Region | Length (bp) | Pi | Alignment reliability | MarkerSeek score | Primer available | Action |
|---|---|---|---|---|---|---|---|
| ycf2-trnL-CAA | IRb | 1336 | 0.0850 | 0.89 | 76.1 | yes | View details |
| ndhB | IRb | 2212 | 0.0015 | 1.00 | 32.9 | no | View details |
| trnV-GAC-ycf15 | IRa | 681 | 0.0205 | 1.00 | 61.6 | yes | View details |
| ndhB | IRa | 2212 | 0.0312 | 1.00 | 62.3 | yes | View details |
| ndhB-trnL-CAA | IRa | 573 | 0.0331 | 1.00 | 75.5 | yes | View details |
| trnL-CAA-trnH-GUG | IRa | 1513 | 0.0974 | 0.91 | 83.5 | no | View details |
| trnH-GUG-psbA | LSC | 197 | 0.0510 | 0.98 | 77.3 | yes | View details |
| ycf15-rps12 | IRa | 972 | 0.0183 | 0.99 | 76.3 | yes | View details |
| rpl32-trnL-UAG | SSC | 915 | 0.0242 | 0.98 | 75.4 | yes | View details |
| trnV-GAC | IRa | 72 | 0.0813 | 1.00 | 73.2 | yes | View details |
| trnC-GCA-petN | LSC | 738 | 0.0203 | 0.97 | 72.1 | yes | View details |
| petN-psbM | LSC | 1155 | 0.0128 | 0.99 | 71.9 | yes | View details |
| ndhF-rpl32 | SSC | 1018 | 0.0200 | 1.00 | 71.1 | yes | View details |
| atpH-atpI | LSC | 966 | 0.0135 | 0.99 | 70.9 | yes | View details |
| trnG-UCC-trnR-UCU | LSC | 136 | 0.0331 | 1.00 | 69.0 | yes | View details |
| rpoB-trnC-GCA | LSC | 1229 | 0.0137 | 0.98 | 68.3 | yes | View details |
| trnV-GAC | IRb | 72 | 0.0012 | 1.00 | 33.0 | no | View details |
| Candidate markers include all hotspot labels marked in the diversity plot, listed in genomic order, plus the top 10 remaining regions by MarkerSeek score. Primer design is attempted for every listed region. Primer available: yes means at least one valid primer pair was found; no means no valid pair passed screening; NA means primer design was skipped. | |||||||
Species
24
Genome length
142–155 kb
Candidate markers
265
Primer pairs
70
Genome-wide nucleotide diversity
Candidate markers
6 hotspot labels from the diversity plot in genomic order, plus the top 11 remaining regions by MarkerSeek score (out of 265 candidates).
Primer pairs
Showing the top 30 of 70 primer pairs (ranked by primer score).
| Primer ID | Label | Forward | Reverse | Amplicon (bp) | Cross-species rate | Score |
|---|---|---|---|---|---|---|
| trnH-GUG-psbA_p1 | trnH-GUG-psbA | GATCCACTTGGCTACATCCG |
GCGCTAACCTTGGTATGGAA |
295–339 | 1.000 | 90.7 |
| trnH-GUG-psbA_p2 | trnH-GUG-psbA | CAATCCACTGCCTTGATCCA |
GCGCTAACCTTGGTATGGAA |
309–353 | 1.000 | 90.7 |
| trnH-GUG-psbA_p3 | trnH-GUG-psbA | AATCCACTGCCTTGATCCAC |
GCGCTAACCTTGGTATGGAA |
308–352 | 1.000 | 90.7 |
| trnH-GUG-psbA_p4 | trnH-GUG-psbA | ACAATCCACTGCCTTGATCC |
GCGCTAACCTTGGTATGGAA |
310–354 | 1.000 | 90.7 |
| trnH-GUG-psbA_p5 | trnH-GUG-psbA | GATCCACTTGGCTACATCCG |
CCTCTAGACCTAGCTGCTGT |
245–289 | 1.000 | 90.4 |
| trnG-UCC-trnR-UCU_p1 | trnG-UCC-trnR-UCU | AGCCTTCCAAGCTAACGATG |
AGAAGACCTCTGTCCTATCCA |
203–236 | 1.000 | 74.3 |
| trnG-UCC-trnR-UCU_p2 | trnG-UCC-trnR-UCU | CCTAGCCTTCCAAGCTAACG |
AGAAGACCTCTGTCCTATCCA |
206–239 | 1.000 | 74.0 |
| trnG-UCC-trnR-UCU_p3 | trnG-UCC-trnR-UCU | AGCCTTCCAAGCTAACGATG |
AGGTTTAGAAGACCTCTGTCCT |
209–242 | 1.000 | 73.5 |
| trnG-UCC-trnR-UCU_p4 | trnG-UCC-trnR-UCU | CCTAGCCTTCCAAGCTAACG |
AGGTTTAGAAGACCTCTGTCCT |
212–245 | 1.000 | 73.1 |
| trnG-UCC-trnR-UCU_p5 | trnG-UCC-trnR-UCU | CCCTAGCCTTCCAAGCTAAC |
AGAAGACCTCTGTCCTATCCA |
207–240 | 1.000 | 71.8 |
| atpH-atpI_p1 | atpH-atpI | ATAACGGAAGCGGCAGAAAT |
ATAGGGGAATCCATGGAGGG |
879–1280 | 1.000 | 88.5 |
| atpH-atpI_p2 | atpH-atpI | TACCTTGACCAACTCCAGGT |
ATAGGGGAATCCATGGAGGG |
935–1336 | 1.000 | 87.6 |
| atpH-atpI_p3 | atpH-atpI | AGCCAATCCAGCAGCAATAA |
ATAGGGGAATCCATGGAGGG |
895–1296 | 1.000 | 87.5 |
| atpH-atpI_p4 | atpH-atpI | AATAACGGAAGCGGCAGAAA |
ATAGGGGAATCCATGGAGGG |
880–1281 | 1.000 | 87.3 |
| atpH-atpI_p5 | atpH-atpI | GCAGTACCTTGACCAACTCC |
ATAGGGGAATCCATGGAGGG |
939–1340 | 1.000 | 87.0 |
| rpoB-trnC-GCA_p1 | rpoB-trnC-GCA | CCACGAACCTACAAAAGCCT |
CCGGATTTGAACTGGGGAAA |
1351–1374 | 1.000 | 86.0 |
| rpoB-trnC-GCA_p2 | rpoB-trnC-GCA | TCCACGAACCTACAAAAGCC |
CCGGATTTGAACTGGGGAAA |
1352–1375 | 1.000 | 86.0 |
| rpoB-trnC-GCA_p3 | rpoB-trnC-GCA | CCACGAACCTACAAAAGCCT |
TTATTGATCAGGCGACACCC |
1369–1392 | 1.000 | 84.0 |
| rpoB-trnC-GCA_p4 | rpoB-trnC-GCA | CCACGAACCTACAAAAGCCT |
AAAAGGATTTGCAGTCCCCC |
1333–1356 | 1.000 | 84.0 |
| rpoB-trnC-GCA_p5 | rpoB-trnC-GCA | TCCACGAACCTACAAAAGCC |
TTATTGATCAGGCGACACCC |
1370–1393 | 1.000 | 83.9 |
| trnC-GCA-petN_p1 | trnC-GCA-petN | TTTCCCCAGTTCAAATCCGG |
AGCCCAAGCGAGACTTACTA |
734–871 | 1.000 | 89.2 |
| trnC-GCA-petN_p2 | trnC-GCA-petN | TTTCCCCAGTTCAAATCCGG |
AGAGTCCACTTCTTCCCCAT |
795–932 | 1.000 | 87.3 |
| trnC-GCA-petN_p3 | trnC-GCA-petN | GGGGGACTGCAAATCCTTTT |
AGCCCAAGCGAGACTTACTA |
752–889 | 1.000 | 86.9 |
| trnC-GCA-petN_p4 | trnC-GCA-petN | GGGGGACTGCAAATCCTTTT |
AGAGTCCACTTCTTCCCCAT |
813–950 | 1.000 | 85.0 |
| trnC-GCA-petN_p5 | trnC-GCA-petN | TTTCCCCAGTTCAAATCCGG |
GCCCAAGCGAGACTTACTAT |
733–870 | 1.000 | 83.3 |
| petN-psbM_p1 | petN-psbM | TAGTAAGTCTCGCTTGGGCT |
TGCTACTGCACTGTTCATCC |
1303–1406 | 1.000 | 86.7 |
| petN-psbM_p2 | petN-psbM | ATGGGGAAGAAGTGGACTCT |
TGCTACTGCACTGTTCATCC |
1242–1345 | 1.000 | 85.4 |
| petN-psbM_p3 | petN-psbM | TAGTAAGTCTCGCTTGGGCT |
GCTACTGCACTGTTCATCCT |
1302–1405 | 1.000 | 84.3 |
| petN-psbM_p4 | petN-psbM | ATGGGGAAGAAGTGGACTCT |
GCTACTGCACTGTTCATCCT |
1241–1344 | 1.000 | 83.0 |
| petN-psbM_p5 | petN-psbM | ATAGTAAGTCTCGCTTGGGC |
TGCTACTGCACTGTTCATCC |
1304–1407 | 1.000 | 80.7 |
Result downloads
Reference species (24)
One GenBank record per species. Use the NCBI button to open the Nucleotide entry for the listed accession.
| Species | Accession | Length (bp) | Link |
|---|---|---|---|
| Peucedanum ampliatum | NC_062055.1 | 147403 | View on NCBI ↗ |
| Peucedanum angelicoides | NC_062054.1 | 142494 | View on NCBI ↗ |
| Peucedanum chujaense | PX549438.1 | 147679 | View on NCBI ↗ |
| Peucedanum dissolutum | OR525844.1 | 154686 | View on NCBI ↗ |
| Peucedanum elegans | NC_070401.1 | 147790 | View on NCBI ↗ |
| Peucedanum franchetii | NC_072976.1 | 148281 | View on NCBI ↗ |
| Peucedanum guangxiense | OR525848.1 | 152969 | View on NCBI ↗ |
| Peucedanum hakuunense | NC_060840.1 | 147426 | View on NCBI ↗ |
| Peucedanum harry-smithii | NC_085567.1 | 147113 | View on NCBI ↗ |
| Peucedanum harry-smithii var. grande | NC_062056.1 | 147046 | View on NCBI ↗ |
| Peucedanum harry-smithii var. subglabrum | OR525852.1 | 147143 | View on NCBI ↗ |
| Peucedanum ledebourielloides | NC_087702.1 | 151015 | View on NCBI ↗ |
| Peucedanum litorale | PX549443.1 | 147691 | View on NCBI ↗ |
| Peucedanum longshengense | NC_062058.1 | 147967 | View on NCBI ↗ |
| Peucedanum mashanense | NC_062057.1 | 154230 | View on NCBI ↗ |
| Peucedanum miroense | PX549445.1 | 148315 | View on NCBI ↗ |
| Peucedanum morisonii | NC_087703.1 | 147105 | View on NCBI ↗ |
| Peucedanum muliense | NC_085181.1 | 148383 | View on NCBI ↗ |
| Peucedanum ostruthium | PP693366.1 | 147692 | View on NCBI ↗ |
| Peucedanum paishanense | PX549444.1 | 147995 | View on NCBI ↗ |
| Peucedanum shanianum | PX398768.1 | 148086 | View on NCBI ↗ |
| Peucedanum tongkangense | PX549448.1 | 147087 | View on NCBI ↗ |
| Peucedanum torilifolium | OR525849.1 | 142433 | View on NCBI ↗ |
| Peucedanum wawrae | NC_067882.1 | 147087 | View on NCBI ↗ |