Hotspot

trnV-GAC

IRa · 139536-139607 · 72 bp

Back to Peucedanum

Pi 0.0813

MarkerSeek score 73.20

Primer available yes

Label trnV-GAC

Feature Metadata

label_name: trnV-GAC
feature_type: tRNA
region: IRa
length_bp: 72
variable_sites: 45
pi: 0.0813
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC_p1	TCAATCCCACGAGCCTCTTA	TAGTGGCGTTGAGTTTCTCG	50.0	50.0	58.1	57.9	211-221 bp	0.916667	79.8
trnV-GAC_p2	TCAATCCCACGAGCCTCTTA	TGAGTTTCTCGACCCTTTGC	50.0	50.0	58.1	58.1	202-212 bp	0.916667	79.1
trnV-GAC_p3	TCAATCCCACGAGCCTCTTA	CTATTTCTCGATGGGGGCAG	50.0	55.0	58.1	57.8	165-175 bp	0.916667	79.1
trnV-GAC_p4	TCAATCCCACGAGCCTCTTA	TCTATTTCTCGATGGGGGCA	50.0	50.0	58.1	58.2	166-176 bp	0.916667	78.9
trnV-GAC_p5	TCAATCCCACGAGCCTCTTA	CGTTGAGTTTCTCGACCCTT	50.0	50.0	58.1	57.8	205-215 bp	0.916667	78.8