Hotspot

ndhB-trnL-CAA

IRa · 145283-145855 · 573 bp

Back to Peucedanum

Pi 0.0331

MarkerSeek score 75.50

Primer available yes

Label ndhB-trnL-CAA

Feature Metadata

label_name: ndhB-trnL-CAA
feature_type: igs
region: IRa
length_bp: 573
variable_sites: 116
pi: 0.0331
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhB-trnL-CAA_p1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	50.0	50.0	57.9	58.0	624-644 bp	0.916667	77.9
ndhB-trnL-CAA_p2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	50.0	50.0	57.9	58.0	622-642 bp	0.916667	77.9
ndhB-trnL-CAA_p3	TGCAATTGCTCAGGATACCC	TACCATTTCACCACCAAGGC	50.0	50.0	57.9	58.1	612-632 bp	0.916667	77.4
ndhB-trnL-CAA_p4	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	50.0	50.0	57.9	58.2	619-639 bp	0.916667	76.4
ndhB-trnL-CAA_p5	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	50.0	50.0	57.9	57.7	623-643 bp	0.916667	75.8