primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	trnH-GUG-psbA	1	GATCCACTTGGCTACATCCG	GCGCTAACCTTGGTATGGAA	20	20	55.0	50.0	58.1	58.0	0.0	0.0	0.161600	121	317	295-339	1.000000	90.7
trnH-GUG-psbA_p2	trnH-GUG-psbA	2	CAATCCACTGCCTTGATCCA	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.165962	121	317	309-353	1.000000	90.7
trnH-GUG-psbA_p3	trnH-GUG-psbA	3	AATCCACTGCCTTGATCCAC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	121	317	308-352	1.000000	90.7
trnH-GUG-psbA_p4	trnH-GUG-psbA	4	ACAATCCACTGCCTTGATCC	GCGCTAACCTTGGTATGGAA	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.167469	121	317	310-354	1.000000	90.7
trnH-GUG-psbA_p5	trnH-GUG-psbA	5	GATCCACTTGGCTACATCCG	CCTCTAGACCTAGCTGCTGT	20	20	55.0	55.0	58.1	57.9	0.0	13.4	0.194818	121	317	245-289	1.000000	90.4
trnG-UCC-trnR-UCU_p1	trnG-UCC-trnR-UCU	1	AGCCTTCCAAGCTAACGATG	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.081443	9820	9955	203-236	1.000000	74.3
trnG-UCC-trnR-UCU_p2	trnG-UCC-trnR-UCU	2	CCTAGCCTTCCAAGCTAACG	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	58.1	56.9	0.0	0.0	2.110280	9820	9955	206-239	1.000000	74.0
trnG-UCC-trnR-UCU_p3	trnG-UCC-trnR-UCU	3	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.164771	9820	9955	209-242	1.000000	73.5
trnG-UCC-trnR-UCU_p4	trnG-UCC-trnR-UCU	4	CCTAGCCTTCCAAGCTAACG	AGGTTTAGAAGACCTCTGTCCT	20	22	55.0	45.5	58.1	57.9	0.0	0.0	2.193608	9820	9955	212-245	1.000000	73.1
trnG-UCC-trnR-UCU_p5	trnG-UCC-trnR-UCU	5	CCCTAGCCTTCCAAGCTAAC	AGAAGACCTCTGTCCTATCCA	20	21	55.0	47.6	57.7	56.9	0.0	0.0	2.385293	9820	9955	207-240	1.000000	71.8
atpH-atpI_p1	atpH-atpI	1	ATAACGGAAGCGGCAGAAAT	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.0	57.9	1.4	21.3	0.104185	13520	14485	879-1280	1.000000	88.5
atpH-atpI_p2	atpH-atpI	2	TACCTTGACCAACTCCAGGT	ATAGGGGAATCCATGGAGGG	20	20	50.0	55.0	57.9	57.9	0.0	21.3	0.178077	13520	14485	935-1336	1.000000	87.6
atpH-atpI_p3	atpH-atpI	3	AGCCAATCCAGCAGCAATAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	57.9	57.9	0.0	21.3	0.214244	13520	14485	895-1296	1.000000	87.5
atpH-atpI_p4	atpH-atpI	4	AATAACGGAAGCGGCAGAAA	ATAGGGGAATCCATGGAGGG	20	20	45.0	55.0	58.2	57.9	1.4	21.3	0.249724	13520	14485	880-1281	1.000000	87.3
atpH-atpI_p5	atpH-atpI	5	GCAGTACCTTGACCAACTCC	ATAGGGGAATCCATGGAGGG	20	20	55.0	55.0	58.2	57.9	0.0	21.3	0.256114	13520	14485	939-1340	1.000000	87.0
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	CCACGAACCTACAAAAGCCT	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.189918	26778	28006	1351-1374	1.000000	86.0
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	TCCACGAACCTACAAAAGCC	CCGGATTTGAACTGGGGAAA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.195241	26778	28006	1352-1375	1.000000	86.0
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	CCACGAACCTACAAAAGCCT	TTATTGATCAGGCGACACCC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.433374	26778	28006	1369-1392	1.000000	84.0
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	CCACGAACCTACAAAAGCCT	AAAAGGATTTGCAGTCCCCC	20	20	50.0	50.0	58.1	58.4	0.0	14.6	0.463281	26778	28006	1333-1356	1.000000	84.0
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	TCCACGAACCTACAAAAGCC	TTATTGATCAGGCGACACCC	20	20	50.0	50.0	58.1	57.7	0.0	0.0	0.438696	26778	28006	1370-1393	1.000000	83.9
trnC-GCA-petN_p1	trnC-GCA-petN	1	TTTCCCCAGTTCAAATCCGG	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.233476	28078	28815	734-871	1.000000	89.2
trnC-GCA-petN_p2	trnC-GCA-petN	2	TTTCCCCAGTTCAAATCCGG	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.1	57.6	0.0	0.0	0.432990	28078	28815	795-932	1.000000	87.3
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTTACTA	20	20	50.0	50.0	58.4	58.2	14.7	0.0	0.506839	28078	28815	752-889	1.000000	86.9
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.706353	28078	28815	813-950	1.000000	85.0
trnC-GCA-petN_p5	trnC-GCA-petN	5	TTTCCCCAGTTCAAATCCGG	GCCCAAGCGAGACTTACTAT	20	20	50.0	50.0	58.1	57.1	0.0	0.0	0.974912	28078	28815	733-870	1.000000	83.3
petN-psbM_p1	petN-psbM	1	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATCC	20	20	50.0	50.0	58.2	57.9	0.0	0.0	0.250489	28906	30060	1303-1406	1.000000	86.7
petN-psbM_p2	petN-psbM	2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATCC	20	20	50.0	50.0	57.6	57.9	0.0	0.0	0.450004	28906	30060	1242-1345	1.000000	85.4
petN-psbM_p3	petN-psbM	3	TAGTAAGTCTCGCTTGGGCT	GCTACTGCACTGTTCATCCT	20	20	50.0	50.0	58.2	57.6	0.0	0.0	0.544620	28906	30060	1302-1405	1.000000	84.3
petN-psbM_p4	petN-psbM	4	ATGGGGAAGAAGTGGACTCT	GCTACTGCACTGTTCATCCT	20	20	50.0	50.0	57.6	57.6	0.0	0.0	0.744134	28906	30060	1241-1344	1.000000	83.0
petN-psbM_p5	petN-psbM	5	ATAGTAAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATCC	20	20	50.0	50.0	57.1	57.9	0.0	0.0	0.991926	28906	30060	1304-1407	1.000000	80.7
ycf2-trnL-CAA_p1	ycf2-trnL-CAA	1	CAGCCCTTTGTCTCTGTGTT	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.034958	92658	93993	798-1967	1.000000	91.7
ycf2-trnL-CAA_p2	ycf2-trnL-CAA	2	CAGCCCTTTGTCTCTGTGTT	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.036329	92658	93993	800-1969	1.000000	91.7
ycf2-trnL-CAA_p3	ycf2-trnL-CAA	3	GTTCTTGCAGAGTGGAACCA	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.040512	92658	93993	922-2091	1.000000	91.7
ycf2-trnL-CAA_p4	ycf2-trnL-CAA	4	GTTCTTGCAGAGTGGAACCA	TGAAATGGTAGACACGCGAG	20	20	50.0	50.0	58.0	58.0	0.0	15.9	0.041882	92658	93993	924-2093	1.000000	91.7
ycf2-trnL-CAA_p5	ycf2-trnL-CAA	5	GGCAAGCGGATCCTTTATGA	AAATGGTAGACACGCGAGAC	20	20	50.0	50.0	58.0	58.0	10.8	15.9	0.040512	92658	93993	975-2144	0.916667	88.3
ndhF-rpl32_p1	ndhF-rpl32	1	GTTGCCGACGGAAAAAGAAG	TGACAAACAGGTTGATGGGG	20	20	50.0	50.0	58.0	58.0	20.5	4.8	0.038458	113459	114476	1469-1628	1.000000	90.3
ndhF-rpl32_p2	ndhF-rpl32	2	AAGCGGGTCGATCAAGTAAC	TGACAAACAGGTTGATGGGG	20	20	50.0	50.0	58.0	58.0	0.0	4.8	0.012015	113459	114476	1647-1806	1.000000	90.2
ndhF-rpl32_p3	ndhF-rpl32	3	CCGTGTGAACCAAAACCCTA	TGACAAACAGGTTGATGGGG	20	20	50.0	50.0	58.0	58.0	0.0	4.8	0.035952	113459	114476	1875-2034	1.000000	89.5
ndhF-rpl32_p4	ndhF-rpl32	4	AAGCGGGTCGATCAAGTAAC	CGGTTGGGTTGAAAGAGACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044177	113459	114476	1874-2099	0.708333	77.7
ndhF-rpl32_p5	ndhF-rpl32	5	AAGCGGGTCGATCAAGTAAC	TGTCGGTTGGGTTGAAAGAG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044177	113459	114476	1877-2102	0.708333	77.7
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGGATATTGGGCAGCCTTA	AGCGTGTCTACCGATTTCAC	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.174643	114639	115553	909-1130	1.000000	89.3
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGGATATTGGGCAGCCTTA	CAGCGTGTCTACCGATTTCA	20	20	50.0	50.0	58.2	58.0	12.4	0.0	0.176014	114639	115553	910-1131	1.000000	89.3
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGGATATTGGGCAGCCTTA	CTCGCACTGCTTCCTAAGAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	114639	115553	930-1151	1.000000	88.6
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGGATATTGGGCAGCCTTA	CTCTCGCACTGCTTCCTAAG	20	20	50.0	55.0	58.2	58.1	12.4	0.0	0.246895	114639	115553	932-1153	1.000000	88.6
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGGATATTGGGCAGCCTTA	TCCTAAGAGCAGCGTGTCTA	20	20	50.0	50.0	58.2	57.9	12.4	0.0	0.291090	114639	115553	919-1140	1.000000	88.3
trnV-GAC_p1	trnV-GAC	1	TCAATCCCACGAGCCTCTTA	TAGTGGCGTTGAGTTTCTCG	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.199685	139536	139607	211-221	0.916667	79.8
trnV-GAC_p2	trnV-GAC	2	TCAATCCCACGAGCCTCTTA	TGAGTTTCTCGACCCTTTGC	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.260406	139536	139607	202-212	0.916667	79.1
trnV-GAC_p3	trnV-GAC	3	TCAATCCCACGAGCCTCTTA	CTATTTCTCGATGGGGGCAG	20	20	50.0	55.0	58.1	57.8	0.0	0.0	0.315237	139536	139607	165-175	0.916667	79.1
trnV-GAC_p4	trnV-GAC	4	TCAATCCCACGAGCCTCTTA	TCTATTTCTCGATGGGGGCA	20	20	50.0	50.0	58.1	58.2	0.0	0.0	0.338116	139536	139607	166-176	0.916667	78.9
trnV-GAC_p5	trnV-GAC	5	TCAATCCCACGAGCCTCTTA	CGTTGAGTTTCTCGACCCTT	20	20	50.0	50.0	58.1	57.8	0.0	10.4	0.289756	139536	139607	205-215	0.916667	78.8
trnV-GAC-ycf15_p1	trnV-GAC-ycf15	1	ACACTCTACCGCTGAGTTAT	GTACCGTGAGAGAAGCAAGG	20	20	45.0	55.0	55.7	58.0	0.0	0.0	2.309211	139608	140288	789-807	0.916667	59.6
trnV-GAC-ycf15_p2	trnV-GAC-ycf15	2	ACACTCTACCGCTGAGTTAT	TGAGAGAAGCAAGGAGGTCA	20	20	45.0	50.0	55.7	58.0	0.0	0.0	2.322336	139608	140288	783-801	0.916667	59.5
trnV-GAC-ycf15_p3	trnV-GAC-ycf15	3	TCTACCGCTGAGTTATATCCCT	GTACCGTGAGAGAAGCAAGG	22	20	45.5	55.0	57.6	58.0	0.0	0.0	2.386262	139608	140288	785-803	0.916667	59.0
trnV-GAC-ycf15_p4	trnV-GAC-ycf15	4	ACACTCTACCGCTGAGTTAT	TACCGTGAGAGAAGCAAGGA	20	20	45.0	50.0	55.7	58.1	0.0	0.0	2.387640	139608	140288	788-806	0.916667	59.0
trnV-GAC-ycf15_p5	trnV-GAC-ycf15	5	TCTACCGCTGAGTTATATCCCT	TGAGAGAAGCAAGGAGGTCA	22	20	45.5	50.0	57.6	58.0	0.0	0.0	2.399387	139608	140288	779-797	0.916667	58.9
ycf15-rps12_p1	ycf15-rps12	1	TCTCCTCGGTCCACAGATAC	GCAACTTTACGTAGGGCAGA	20	20	55.0	50.0	57.9	57.9	0.0	0.0	0.143182	140481	141452	1089-1131	0.916667	79.5
ycf15-rps12_p2	ycf15-rps12	2	TTTCTCCTCGGTCCACAGAT	GCAACTTTACGTAGGGCAGA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.150365	140481	141452	1091-1133	0.916667	79.4
ycf15-rps12_p3	ycf15-rps12	3	TCTCCTCGGTCCACAGATAC	CGTAGGGCAGAGTTTGGTTT	20	20	55.0	50.0	57.9	58.1	0.0	0.0	0.162023	140481	141452	1080-1122	0.916667	79.4
ycf15-rps12_p4	ycf15-rps12	4	TTTCTCCTCGGTCCACAGAT	CGTAGGGCAGAGTTTGGTTT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.169206	140481	141452	1082-1124	0.916667	79.3
ycf15-rps12_p5	ycf15-rps12	5	AAATGTAAGACTGGCGCCAA	GCAACTTTACGTAGGGCAGA	20	20	45.0	50.0	58.1	57.9	32.1	0.0	0.184663	140481	141452	1068-1095	0.916667	79.3
ndhB_p1	ndhB	1	TGGATGCAGTTACTAATTCACG	TTTGGGCGGAACAGATCTAC	22	20	40.9	50.0	56.5	57.6	0.0	0.0	3.863870	143071	145282	2312-2312	0.083333	12.4
ndhB_p2	ndhB	2	TGGATGCAGTTACTAATTCACG	TTGGGCGGAACAGATCTACT	22	20	40.9	50.0	56.5	58.4	0.0	0.0	3.898776	143071	145282	2311-2311	0.083333	12.1
ndhB_p3	ndhB	3	TGGATGCAGTTACTAATTCACG	TGGGCGGAACAGATCTACTA	22	20	40.9	50.0	56.5	57.6	0.0	0.0	3.914688	143071	145282	2310-2310	0.083333	12.0
ndhB_p4	ndhB	4	TTGGATGCAGTTACTAATTCACG	TTTGGGCGGAACAGATCTAC	23	20	39.1	50.0	57.2	57.6	0.0	0.0	4.191277	143071	145282	2313-2313	0.083333	9.8
ndhB_p5	ndhB	5	TTGGATGCAGTTACTAATTCACG	TTGGGCGGAACAGATCTACT	23	20	39.1	50.0	57.2	58.4	0.0	0.0	4.226183	143071	145282	2312-2312	0.083333	9.5
ndhB-trnL-CAA_p1	ndhB-trnL-CAA	1	TGCAATTGCTCAGGATACCC	GTCTCGCGTGTCTACCATTT	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.064968	145283	145855	624-644	0.916667	77.9
ndhB-trnL-CAA_p2	ndhB-trnL-CAA	2	TGCAATTGCTCAGGATACCC	CTCGCGTGTCTACCATTTCA	20	20	50.0	50.0	57.9	58.0	29.4	5.8	0.066338	145283	145855	622-642	0.916667	77.9
ndhB-trnL-CAA_p3	ndhB-trnL-CAA	3	TGCAATTGCTCAGGATACCC	TACCATTTCACCACCAAGGC	20	20	50.0	50.0	57.9	58.1	29.4	0.0	0.139265	145283	145855	612-632	0.916667	77.4
ndhB-trnL-CAA_p4	ndhB-trnL-CAA	4	TGCAATTGCTCAGGATACCC	GCGTGTCTACCATTTCACCA	20	20	50.0	50.0	57.9	58.2	29.4	0.0	0.253803	145283	145855	619-639	0.916667	76.4
ndhB-trnL-CAA_p5	ndhB-trnL-CAA	5	TGCAATTGCTCAGGATACCC	TCTCGCGTGTCTACCATTTC	20	20	50.0	50.0	57.9	57.7	29.4	5.8	0.330507	145283	145855	623-643	0.916667	75.8