Hotspot

ndhF-rpl32

SSC · 113459-114476 · 1018 bp

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Pi 0.02

MarkerSeek score 71.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1018
variable_sites: 102
pi: 0.02
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGACGGAAAAAGAAG	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1469-1628 bp	1.0	90.3
ndhF-rpl32_p2	AAGCGGGTCGATCAAGTAAC	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1647-1806 bp	1.0	90.2
ndhF-rpl32_p3	CCGTGTGAACCAAAACCCTA	TGACAAACAGGTTGATGGGG	50.0	50.0	58.0	58.0	1875-2034 bp	1.0	89.5
ndhF-rpl32_p4	AAGCGGGTCGATCAAGTAAC	CGGTTGGGTTGAAAGAGACA	50.0	50.0	58.0	58.0	1874-2099 bp	0.708333	77.7
ndhF-rpl32_p5	AAGCGGGTCGATCAAGTAAC	TGTCGGTTGGGTTGAAAGAG	50.0	50.0	58.0	58.0	1877-2102 bp	0.708333	77.7