Hotspot

accD

LSC · 56956-58548 · 1593 bp

Back to Lactuca

Pi 0.0142

MarkerSeek score 62.40

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1593
variable_sites: 135
pi: 0.0142
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TGATATGCTTATTCTGATAGGAA	ACAAAGAAAACCCCATTCGTC	30.4	42.9	52.5	57.0	1642-1771 bp	0.944444	48.3
accD_p2	TTGATATGCTTATTCTGATAGGA	ACAAAGAAAACCCCATTCGTC	30.4	42.9	52.5	57.0	1643-1772 bp	0.944444	48.3
accD_p3	TGATATGCTTATTCTGATAGGAA	CAACAAAGAAAACCCCATTCGT	30.4	40.9	52.5	57.9	1644-1773 bp	0.944444	48.3
accD_p4	TTGATATGCTTATTCTGATAGGA	CAACAAAGAAAACCCCATTCGT	30.4	40.9	52.5	57.9	1645-1774 bp	0.944444	48.3
accD_p5	TGATATGCTTATTCTGATAGGAAA	ACAAAGAAAACCCCATTCGTC	29.2	42.9	53.4	57.0	1642-1771 bp	0.944444	48.3