Hotspot

ndhF-rpl32

SSC · 111348-112397 · 1050 bp

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Pi 0.0195
MarkerSeek score 75.90
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1050
variable_sites
149
pi
0.0195
unique_haplotype_count
30
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GTTGCCGTCGGAAAAAGAAG TGACAAAGCTTTTAACGCCG 50.0 45.0 58.0 57.6 1238-1344 bp 1.0 88.6
ndhF-rpl32_p2 TGCCGTCGGAAAAAGAAGAA TGACAAAGCTTTTAACGCCG 45.0 45.0 58.0 57.6 1236-1342 bp 1.0 88.5
ndhF-rpl32_p3 GTTGCCGTCGGAAAAAGAAG CCGCCCAATATCCTTTCCTT 50.0 50.0 58.0 57.6 1221-1327 bp 0.972222 87.7
ndhF-rpl32_p4 TGCCGTCGGAAAAAGAAGAA CCGCCCAATATCCTTTCCTT 45.0 50.0 58.0 57.6 1219-1325 bp 0.972222 87.6
ndhF-rpl32_p5 TTGCCGTCGGAAAAAGAAGA CCGCCCAATATCCTTTCCTT 45.0 50.0 58.0 57.6 1220-1326 bp 0.972222 87.6