Hotspot

trnM-atpE

LSC · 52168-52379 · 212 bp

Back to Lactuca

Pi 0.0167

MarkerSeek score 66.10

Primer available yes

Label trnM-atpE

Feature Metadata

label_name: trnM-atpE
feature_type: igs
region: LSC
length_bp: 212
variable_sites: 23
pi: 0.0167
unique_haplotype_count: 19
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-atpE_p1	TATTGCTTTCATACGGCGGG	CAAATCTAGCTCTCCGACGG	50.0	55.0	58.4	57.9	310-369 bp	1.0	86.4
trnM-atpE_p2	TATTGCTTTCATACGGCGGG	GGCAAATCTAGCTCTCCGAC	50.0	55.0	58.4	58.1	312-371 bp	1.0	86.2
trnM-atpE_p3	TATTGCTTTCATACGGCGGG	GAGGCAAATCTAGCTCTCCG	50.0	55.0	58.4	57.9	314-373 bp	1.0	86.1
trnM-atpE_p4	TATTGCTTTCATACGGCGGG	GGGTAGAGGCTATCAATGCG	50.0	55.0	58.4	57.9	285-339 bp	0.972222	85.2
trnM-atpE_p5	TATTGCTTTCATACGGCGGG	CGGGTAGAGGCTATCAATGC	50.0	55.0	58.4	57.9	286-340 bp	0.972222	85.1