Hotspot

ndhC-trnV-UAC

LSC · 50235-51266 · 1032 bp

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Pi 0.013
MarkerSeek score 66.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1032
variable_sites
99
pi
0.013
unique_haplotype_count
32
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 ATCATATTCATAAAGCAGAAACA CCGAGAAAGTCTACGGTTCG 26.1 55.0 52.2 58.1 1072-1141 bp 1.0 51.3
ndhC-trnV-UAC_p2 TCATATTCATAAAGCAGAAACAT CCGAGAAAGTCTACGGTTCG 26.1 55.0 52.2 58.1 1071-1140 bp 1.0 51.3
ndhC-trnV-UAC_p3 ATCATATTCATAAAGCAGAAACAT CCGAGAAAGTCTACGGTTCG 25.0 55.0 52.9 58.1 1072-1141 bp 1.0 51.3
ndhC-trnV-UAC_p4 ATCATATTCATAAAGCAGAAACA GTTCGAGTCCGTATAGCCCT 26.1 55.0 52.2 58.7 1057-1126 bp 1.0 51.3
ndhC-trnV-UAC_p5 TCATATTCATAAAGCAGAAACAT GTTCGAGTCCGTATAGCCCT 26.1 55.0 52.2 58.7 1056-1125 bp 1.0 51.3