Hotspot

ycf3-trnS

LSC · 44097-44981 · 885 bp

Back to Lactuca

Pi 0.0138

MarkerSeek score 77.10

Primer available yes

Label ycf3-trnS

Feature Metadata

label_name: ycf3-trnS
feature_type: igs
region: LSC
length_bp: 885
variable_sites: 87
pi: 0.0138
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-trnS_p1	CATTTATCCGCGATCTAGGCA	TTCCAATGCTACGCCTTCAA	47.6	45.0	58.0	57.8	635-995 bp	1.0	81.3
ycf3-trnS_p2	CATTTATCCGCGATCTAGGCA	GGATTCGAACCCTCGGTAAG	47.6	55.0	58.0	57.8	673-1033 bp	1.0	80.8
ycf3-trnS_p3	CATTTATCCGCGATCTAGGCA	AGTTCCAATGCTACGCCTTC	47.6	50.0	58.0	58.3	637-997 bp	1.0	80.8
ycf3-trnS_p4	CATTTATCCGCGATCTAGGCA	AGGTGAAGGTACGGAAAGGA	47.6	50.0	58.0	58.0	1010-1045 bp	0.222222	40.6
ycf3-trnS_p5	CATTTATCCGCGATCTAGGCA	ACGGAAAGGAAAGAGAGGGA	47.6	50.0	58.0	58.0	1000-1035 bp	0.222222	40.6