Hotspot

trnK-UUU-rps16

LSC · 4374-5187 · 814 bp

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Pi 0.0169
MarkerSeek score 82.40
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
814
variable_sites
90
pi
0.0169
unique_haplotype_count
34
primer_available
yes
alignment_reliability
0.99
markerseek_score
82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GGTATGGATGAATCCCTCGC TTCCTTGAAAAAGGCGCTCA 55.0 45.0 57.9 58.3 975-1042 bp 1.0 88.3
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 920-987 bp 1.0 88.0
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 58.2 58.3 919-986 bp 1.0 88.0
trnK-UUU-rps16_p4 TCAGTCGTGGTCTTCCAAAC TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1004-1071 bp 1.0 87.0
trnK-UUU-rps16_p5 CAGTCGTGGTCTTCCAAACT TTCCTTGAAAAAGGCGCTCA 50.0 45.0 57.8 58.3 1003-1070 bp 1.0 87.0