Hotspot

rps16

LSC · 5302-6506 · 1205 bp

Back to Gilbertiodendron

Pi 0.0169

MarkerSeek score 57.80

Primer available yes

Label rps16

Feature Metadata

label_name: rps16
feature_type: gene
region: LSC
length_bp: 1205
variable_sites: 27
pi: 0.0169
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16_p1	TCCACACCCTCTTTTGTTTCT	TGGCTGCAAACCAGAGAAAT	42.9	45.0	57.3	58.0	1242-1256 bp	1.0	69.4
rps16_p2	TCCACACCCTCTTTTGTTTCT	GGCTGCAAACCAGAGAAATG	42.9	50.0	57.3	57.6	1241-1255 bp	1.0	66.6
rps16_p3	TCCACACCCTCTTTTGTTTCTT	TGGCTGCAAACCAGAGAAAT	40.9	45.0	58.0	58.0	1242-1256 bp	1.0	66.6
rps16_p4	ATCCACACCCTCTTTTGTTTCT	TGGCTGCAAACCAGAGAAAT	40.9	45.0	57.8	58.0	1243-1257 bp	1.0	65.3
rps16_p5	TCCACACCCTCTTTTGTTTC	TGGCTGCAAACCAGAGAAAT	45.0	45.0	55.8	58.0	1242-1256 bp	1.0	65.0