Hotspot

ndhD-psaC

SSC · 121470-121596 · 127 bp

Back to Gilbertiodendron

Pi 0.021

MarkerSeek score 70.50

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 127
variable_sites: 4
pi: 0.021
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AATGAACCCGCGGATATTGG	CCGACGGATTTCTTGAGTGT	50.0	50.0	58.4	57.9	255-258 bp	1.0	81.4
ndhD-psaC_p2	AATGAACCCGCGGATATTGG	TCCGACGGATTTCTTGAGTG	50.0	50.0	58.4	57.6	256-259 bp	1.0	79.2
ndhD-psaC_p3	AATGAACCCGCGGATATTGG	TGTCCGACGGATTTCTTGAG	50.0	50.0	58.4	57.6	258-261 bp	1.0	79.2
ndhD-psaC_p4	AATGAACCCGCGGATATTGG	CTGTCCGACGGATTTCTTGA	50.0	50.0	58.4	57.6	259-262 bp	1.0	79.1
ndhD-psaC_p5	AATGAACCCGCGGATATTGG	GTCCGACGGATTTCTTGAGT	50.0	50.0	58.4	57.6	257-260 bp	1.0	79.1