Hotspot

rpl32-trnL-UAG

SSC · 117221-118535 · 1315 bp

Back to Gilbertiodendron

Pi 0.0228

MarkerSeek score 65.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1315
variable_sites: 43
pi: 0.0228
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TCCACGGGGAACTCAAAAAG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1366-1398 bp	1.0	86.3
rpl32-trnL-UAG_p2	TCCACGGGGAACTCAAAAAG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1367-1399 bp	1.0	86.3
rpl32-trnL-UAG_p3	CGACCGGTCTGATTCCATTT	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	1857-1885 bp	1.0	85.2
rpl32-trnL-UAG_p4	CGACCGGTCTGATTCCATTT	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	1858-1886 bp	1.0	85.2
rpl32-trnL-UAG_p5	CGACCGGTCTGATTCCATTT	TGCCTTTTCCCAACGAATCA	50.0	45.0	58.0	58.0	2128-2162 bp	1.0	84.7