Hotspot

trnR-ACG-trnN-GUU

IRb · 112253-112891 · 639 bp

Back to Gilbertiodendron

Pi 0.0245

MarkerSeek score 76.10

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 639
variable_sites: 22
pi: 0.0245
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.8	58.1	810-840 bp	1.0	80.7
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	733-763 bp	1.0	80.6
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	720-750 bp	1.0	80.6
trnR-ACG-trnN-GUU_p4	GAACAGATCCAGTGGAGACG	TGGGTTGAGAACGGGAATTG	55.0	50.0	57.7	58.1	847-877 bp	1.0	80.0
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	GGGTTGAGAACGGGAATTGA	55.0	50.0	57.8	57.8	809-839 bp	1.0	79.9