Hotspot

rps3-rps19

LSC · 87003-87703 · 701 bp

Back to Gilbertiodendron

Pi 0.0407

MarkerSeek score 79.30

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 701
variable_sites: 37
pi: 0.0407
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	ACCAAGTCTGAAACCGAGTG	TCTAAACTTTCGGGGACACG	50.0	50.0	57.8	57.6	705-999 bp	1.0	82.1
rps3-rps19_p2	CCAAGTCTGAAACCGAGTGG	TCTAAACTTTCGGGGACACG	55.0	50.0	58.5	57.6	704-998 bp	1.0	80.1
rps3-rps19_p3	CAAGTCTGAAACCGAGTGGA	TCTAAACTTTCGGGGACACG	50.0	50.0	57.5	57.6	703-997 bp	1.0	79.8
rps3-rps19_p4	ACCAAGTCTGAAACCGAGTG	GGGACACGCCAAAAACGATA	50.0	50.0	57.8	58.8	693-987 bp	1.0	79.0
rps3-rps19_p5	ACCAAGTCTGAAACCGAGTG	GGACACGCCAAAAACGATAA	50.0	45.0	57.8	57.0	692-986 bp	1.0	77.9