Hotspot

accD-psaI

LSC · 62074-62591 · 518 bp

Back to Gilbertiodendron

Pi 0.0341

MarkerSeek score 69.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 518
variable_sites: 24
pi: 0.0341
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGGTTCACAAACGGCTGAAT	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	730-787 bp	1.0	84.9
accD-psaI_p2	AACGCCTACATTGCATTTGC	CCATTGCAATTGCCGGAAAT	45.0	45.0	58.0	58.0	795-852 bp	1.0	84.7
accD-psaI_p3	AACGCCTACATTGCATTTGC	AACGACATCACGATCCCTTG	45.0	50.0	58.0	58.0	1232-1289 bp	1.0	83.3
accD-psaI_p4	AGGTTCACAAACGGCTGAAT	AACGACATCACGATCCCTTG	45.0	50.0	58.0	58.0	1167-1224 bp	1.0	83.3
accD-psaI_p5	TGATTCGGAAGAGGAACCCT	AACGACATCACGATCCCTTG	50.0	50.0	58.0	58.0	1647-1704 bp	1.0	82.3