Hotspot

atpB-rbcL

LSC · 57492-58277 · 786 bp

Back to Gilbertiodendron

Pi 0.0233

MarkerSeek score 67.10

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 786
variable_sites: 24
pi: 0.0233
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	TTTCAAGTGCGGAAACCTCA	AACACCAGCTTTGAACCCAA	45.0	45.0	58.0	58.1	876-897 bp	1.0	83.5
atpB-rbcL_p2	TTTCAAGTGCGGAAACCTCA	GCTTTGAACCCAACACTTGC	45.0	50.0	58.0	58.4	869-890 bp	1.0	81.2
atpB-rbcL_p3	TTCAAGTGCGGAAACCTCAG	AACACCAGCTTTGAACCCAA	50.0	45.0	58.4	58.1	875-896 bp	1.0	80.6
atpB-rbcL_p4	GGAAACCTCAGGACCAGAAG	AACACCAGCTTTGAACCCAA	55.0	45.0	57.5	58.1	866-887 bp	1.0	80.0
atpB-rbcL_p5	TTTCAAGTGCGGAAACCTCA	CCAGCTTTGAACCCAACACT	45.0	50.0	58.0	58.6	872-893 bp	1.0	79.8