Hotspot

ndhC-trnV-UAC

LSC · 53816-54379 · 564 bp

Back to Gilbertiodendron

Pi 0.025

MarkerSeek score 63.90

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 564
variable_sites: 18
pi: 0.025
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	AGAGAAGCGGCCATAAACTG	TGATCTAGGCCAGAAGCAGA	50.0	50.0	58.0	57.8	1331-1399 bp	1.0	82.9
ndhC-trnV-UAC_p2	AGAGAAGCGGCCATAAACTG	GCCTGACAAAAGGTTCGGTA	50.0	50.0	58.0	58.1	1586-1654 bp	1.0	82.8
ndhC-trnV-UAC_p3	AGAGAAGCGGCCATAAACTG	GCACAAGGGCTATAGCTCAG	50.0	55.0	58.0	58.1	1742-1810 bp	1.0	82.7
ndhC-trnV-UAC_p4	TTGTTACTGTGCCTGCTGTT	GCCTGACAAAAGGTTCGGTA	45.0	50.0	57.9	58.1	1718-1786 bp	1.0	82.3
ndhC-trnV-UAC_p5	TTGTTACTGTGCCTGCTGTT	GCACAAGGGCTATAGCTCAG	45.0	55.0	57.9	58.1	1874-1942 bp	1.0	82.3