Hotspot

rps4-trnT-UGU

LSC · 48871-49296 · 426 bp

Back to Gilbertiodendron

Pi 0.0269

MarkerSeek score 71.10

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 426
variable_sites: 16
pi: 0.0269
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	TAAAGACCCCAGACGACGTA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.8	57.9	539-589 bp	1.0	82.9
rps4-trnT-UGU_p2	GAAACGAGGCCCTCTGTAAC	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	512-562 bp	1.0	82.6
rps4-trnT-UGU_p3	TAAAGACCCCAGACGACGTA	ATGGTCATCGGTTCGATTCC	50.0	50.0	57.8	57.8	507-557 bp	1.0	82.4
rps4-trnT-UGU_p4	TTTTTGAAACGAGGCCCTCT	CTCAGAGGTTAGAGCATCGC	45.0	55.0	57.6	57.9	517-567 bp	1.0	81.9
rps4-trnT-UGU_p5	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	580-630 bp	1.0	81.7